Literature summary for 1.14.13.61 extracted from

Martins, B.M.; Svetlitchnaia, T.; Dobbek, H.
2-Oxoquinoline 8-monooxygenase oxygenase component: active site modulation by Rieske-[2Fe-2S] center oxidation/reduction (2005), Structure, 13, 817-824.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, hanging drop vapour diffusion method, 50 mg/ml protein in 10 mM Tris-HCl, pH 8.0, is mixed with reservoir solution, containing 3335% PEG 400, 200 mM ammonium tartrate, pH 6.5, in a 3:2 ratio with a resulting pH of 7.0, at aerobic conditions, 3 days at 17°C, under anaerobic conditions a reservoir solution containing 2930% PEG 400, 200 mM ammonium tartrate, 5 mM Na-dithionite, pH 6.5, is mixed with protein reduced by 5 mM Na-dithionite in a ratio of 2:2 at 15°C, X-ray diffraction structure determination and analysis at 2.5 A resolution	Pseudomonas putida

Crystallization (Comment)

Organism

purified enzyme, hanging drop vapour diffusion method, 50 mg/ml protein in 10 mM Tris-HCl, pH 8.0, is mixed with reservoir solution, containing 3335% PEG 400, 200 mM ammonium tartrate, pH 6.5, in a 3:2 ratio with a resulting pH of 7.0, at aerobic conditions, 3 days at 17°C, under anaerobic conditions a reservoir solution containing 2930% PEG 400, 200 mM ammonium tartrate, 5 mM Na-dithionite, pH 6.5, is mixed with protein reduced by 5 mM Na-dithionite in a ratio of 2:2 at 15°C, X-ray diffraction structure determination and analysis at 2.5 A resolution

Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme is a Rieske non-heme iron oxygenase, an active site Rieske-[2Fe-2S] center is involved in the enzyme complex, structure analysis of oxidized, reduced, and substrate bound states, overview	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
quinolin-2-ol + NADH + O2	Pseudomonas putida	the N-heteroaromatic compound quinoline acts as sole source of carbon, nitrogen, and energy in the aerobic soil bacterium	quinolin-2,8-diol + NAD+ + H2O	-	?
quinolin-2-ol + NADH + O2	Pseudomonas putida 86	the N-heteroaromatic compound quinoline acts as sole source of carbon, nitrogen, and energy in the aerobic soil bacterium	quinolin-2,8-diol + NAD+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-
Pseudomonas putida 86	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme by ion exchange and hydrophobic interaction chromatography, and gel filtration	Pseudomonas putida

Reaction

Reaction	Comment	Organism	Reaction ID
quinolin-2-ol + NADH + H+ + O2 = quinolin-2,8-diol + NAD+ + H2O	active site modulation by Rieske-[2Fe-2S] center oxidation/reduction	Pseudomonas putida	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
quinolin-2-ol + NADH + O2	the N-heteroaromatic compound quinoline acts as sole source of carbon, nitrogen, and energy in the aerobic soil bacterium	Pseudomonas putida	quinolin-2,8-diol + NAD+ + H2O	-	?
quinolin-2-ol + NADH + O2	the mononuclear Fe2+ ion and can open a pathway for dioxygen to bind in the substrate-containing active site, substrate binding structure, overview	Pseudomonas putida	quinolin-2,8-diol + NAD+ + H2O	-	?
quinolin-2-ol + NADH + O2	the N-heteroaromatic compound quinoline acts as sole source of carbon, nitrogen, and energy in the aerobic soil bacterium	Pseudomonas putida 86	quinolin-2,8-diol + NAD+ + H2O	-	?
quinolin-2-ol + NADH + O2	the mononuclear Fe2+ ion and can open a pathway for dioxygen to bind in the substrate-containing active site, substrate binding structure, overview	Pseudomonas putida 86	quinolin-2,8-diol + NAD+ + H2O	-	?

Subunits

Subunits	Comment	Organism
More	detailed enzyme structure analysis in oxidized, reduced, and substrate bound states, from cyrstal structure, overview	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
2-oxoquinoline 8-monooxygenase oxygenase	-	Pseudomonas putida
OMO	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Pseudomonas putida