Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Pseudomonas putida |
70000 | - |
1 * 70000, SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
ORC | - |
Pseudomonas putida ORC | - |
ORC | - |
Purification (Comment) | Organism |
---|---|
ORC, using protamine sulfate treatment, column chromatography on DEAE-cellulose, Sephadex G-100, Sephadex G-75 and a second DEAE-cellulose column chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.7 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chlorophenol + NADH + O2 | 33% of activity with orcinol | Pseudomonas putida | ? | - |
? | |
3-cresol + NADH + O2 | 11-14% of the activity with orcinol | Pseudomonas putida | 3-methylcatechol + NAD+ + H2O | - |
? | |
3-cresol + NADH + O2 | 11-14% of the activity with orcinol | Pseudomonas putida ORC | 3-methylcatechol + NAD+ + H2O | - |
? | |
3-ethylphenol + NADH + O2 | 30% of activity with orcinol | Pseudomonas putida | ? | - |
? | |
3-trifluoromethylphenol + NADH + O2 | 94% of activity with orcinol | Pseudomonas putida | ? | - |
? | |
3-trifluoromethylphenol + NADH + O2 | 94% of activity with orcinol | Pseudomonas putida ORC | ? | - |
? | |
additional information | - |
Pseudomonas putida | ? | - |
? | |
additional information | - |
Pseudomonas putida ORC | ? | - |
? | |
orcinol + NADH + O2 | - |
Pseudomonas putida | 2,3,5-trihydroxytoluene + NAD+ + H2O | - |
? | |
orcinol + NADH + O2 | - |
Pseudomonas putida ORC | 2,3,5-trihydroxytoluene + NAD+ + H2O | - |
? | |
resorcinol + NADH + O2 | 23% of activity with orcinol | Pseudomonas putida | hydroxyquinol + NAD+ + H2O | - |
? | |
resorcinol + NADH + O2 | 23% of activity with orcinol | Pseudomonas putida ORC | hydroxyquinol + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Pseudomonas putida | |
FAD | 1 mol of FAD per mol of protein | Pseudomonas putida | |
NADH | - |
Pseudomonas putida | |
NADPH | - |
Pseudomonas putida |