Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Carbonylcyanide-m-chlorophenylhydrazone | - |
Escherichia coli | |
Carbonylcyanide-p-fluoromethoxyphenylhydrazone | - |
Escherichia coli | |
Cinnamylidene | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NADPH | recombinant enzyme form IucD398, with a deletion of 47 amino acids in the N-terminus | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
recombinant enzyme form IucD398, with a deletion of 47 amino acids in the N-terminus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.168 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, medium of ionic strength 0.25 or higher, recombinant enzyme form IacD398, stable for 1 month | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-Aminoethyl-L-Cys + NADPH + O2 | - |
Escherichia coli | ? | - |
? | |
L-Lys + NADH + O2 | with lower efficiency than NADPH, recombinant enzyme form IucD398, with a deletion of 47 amino acids in the N-terminus | Escherichia coli | ? | - |
? | |
L-Lys + NADPH + O2 | - |
Escherichia coli | N6-Hydroxy-L-Lys + NADP+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | Km: 0.0051 mM | Escherichia coli | |
FAD | requires FAD | Escherichia coli | |
NADH | recombinant enzyme form IucD398, with a deletion of 47 amino acids in the N-terminus | Escherichia coli | |
NADPH | required | Escherichia coli |