BRENDA - Enzyme Database show
show all sequences of 1.14.13.54

Studies on steroid monooxygenase from Cylindrocarpon radicicola ATCC 11011. Purification and characterization

Itagaki, E.; J. Biochem. 99, 815-824 (1986)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0004
-
progesterone
-
Ilyonectria destructans
0.0043
-
NADPH
with progesterone and O2 as cosubstrates
Ilyonectria destructans
0.07
-
O2
with progesterone and NADPH as cosubstrates
Ilyonectria destructans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
2 * 56000, SDS-PAGE
Ilyonectria destructans
116000
-
gel filtration, gel electrophoresis
Ilyonectria destructans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ilyonectria destructans
-
ATTC 11011
-
Ilyonectria destructans ATTC 11011
-
ATTC 11011
-
Purification (Commentary)
Commentary
Organism
-
Ilyonectria destructans
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Storage Stability
Storage Stability
Organism
-80°C, Tris buffer, stable for more than 6 months, enzyme in crude extract
Ilyonectria destructans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-Pregnadiene-17alpha,21-diol-3,11,20-trione + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
11beta-Hydroxyprogesterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
11beta-Hydroxyprogesterone + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
?
-
-
-
-
17alpha-Hydroxyprogesterone + NADPH + O2
-
7390
Ilyonectria destructans
androstenedione + acetate + NADP+ + H2O
-
-
-
-
17alpha-Hydroxyprogesterone + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
androstenedione + acetate + NADP+ + H2O
-
-
-
-
18-Hydroxydeoxycorticosterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
21-Deoxycortisol + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
Aldosterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
androstenedione + NADPH + O2
-
7390
Ilyonectria destructans
3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
-
7390
Ilyonectria destructans
-
androstenedione + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
-
7390
Ilyonectria destructans ATTC 11011
-
Corticosterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
Cortisol + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
Cortisone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
deoxycorticosterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
deoxycorticosterone + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
?
-
-
-
-
Progesterone + NADPH + O2
-
7390
Ilyonectria destructans
Testosterone acetate + NADP+ + H2O
-
7390
Ilyonectria destructans
-
Progesterone + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
Testosterone acetate + NADP+ + H2O
-
7390
Ilyonectria destructans ATTC 11011
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 56000, SDS-PAGE
Ilyonectria destructans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
progesterone monooxygenase reaction
Ilyonectria destructans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
pH 6.0: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, progesterone monooxygenase activity
Ilyonectria destructans
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
enzyme contains 1.6 mol of FAD per mol of enzyme
Ilyonectria destructans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
enzyme contains 1.6 mol of FAD per mol of enzyme
Ilyonectria destructans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0004
-
progesterone
-
Ilyonectria destructans
0.0043
-
NADPH
with progesterone and O2 as cosubstrates
Ilyonectria destructans
0.07
-
O2
with progesterone and NADPH as cosubstrates
Ilyonectria destructans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56000
-
2 * 56000, SDS-PAGE
Ilyonectria destructans
116000
-
gel filtration, gel electrophoresis
Ilyonectria destructans
Purification (Commentary) (protein specific)
Commentary
Organism
-
Ilyonectria destructans
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, Tris buffer, stable for more than 6 months, enzyme in crude extract
Ilyonectria destructans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,4-Pregnadiene-17alpha,21-diol-3,11,20-trione + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
11beta-Hydroxyprogesterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
11beta-Hydroxyprogesterone + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
?
-
-
-
-
17alpha-Hydroxyprogesterone + NADPH + O2
-
7390
Ilyonectria destructans
androstenedione + acetate + NADP+ + H2O
-
-
-
-
17alpha-Hydroxyprogesterone + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
androstenedione + acetate + NADP+ + H2O
-
-
-
-
18-Hydroxydeoxycorticosterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
21-Deoxycortisol + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
Aldosterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
androstenedione + NADPH + O2
-
7390
Ilyonectria destructans
3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
-
7390
Ilyonectria destructans
-
androstenedione + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
-
7390
Ilyonectria destructans ATTC 11011
-
Corticosterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
Cortisol + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
Cortisone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
deoxycorticosterone + NADPH + O2
-
7390
Ilyonectria destructans
?
-
-
-
-
deoxycorticosterone + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
?
-
-
-
-
Progesterone + NADPH + O2
-
7390
Ilyonectria destructans
Testosterone acetate + NADP+ + H2O
-
7390
Ilyonectria destructans
-
Progesterone + NADPH + O2
-
7390
Ilyonectria destructans ATTC 11011
Testosterone acetate + NADP+ + H2O
-
7390
Ilyonectria destructans ATTC 11011
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 56000, SDS-PAGE
Ilyonectria destructans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
progesterone monooxygenase reaction
Ilyonectria destructans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
pH 6.0: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, progesterone monooxygenase activity
Ilyonectria destructans
Other publictions for EC 1.14.13.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728352
Swizdor
Baeyer-Villiger oxidation of s ...
Penicillium lanosocoeruleum
Molecules
18
13812-13822
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727906
Franceschini
Exploring the structural basis ...
Rhodococcus rhodochrous
J. Biol. Chem.
287
22626-22634
2012
-
-
1
1
5
-
-
5
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
6
-
-
-
2
-
-
-
-
-
1
2
1
5
-
-
-
-
5
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
11
11
693956
Virus
Molecular evolution of a stero ...
Bacillus megaterium
Lipids
43
1133-1141
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
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2
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1
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1
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-
2
-
-
-
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-
-
-
-
-
-
-
-
-
659076
Morii
Steroid monooxygenase of Rhodo ...
Rhodococcus rhodochrous
J. Biochem.
126
624-631
1999
-
-
1
-
-
-
-
2
-
-
3
1
-
5
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
-
3
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7389
Itagaki
Studies on steroid monooxygena ...
Ilyonectria destructans, Ilyonectria destructans ATTC 11011
J. Biochem.
99
825-832
1986
-
-
-
-
-
-
5
5
-
-
-
-
-
5
-
-
-
-
-
-
1
-
10
-
1
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
-
-
-
-
-
-
-
-
1
-
10
-
1
-
1
-
2
1
-
-
-
-
-
-
-
-
7390
Itagaki
Studies on steroid monooxygena ...
Ilyonectria destructans, Ilyonectria destructans ATTC 11011
J. Biochem.
99
815-824
1986
-
-
-
-
-
-
-
3
-
-
2
-
-
5
-
-
1
-
-
1
-
1
17
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
1
-
1
17
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-