BRENDA - Enzyme Database show
show all sequences of 1.14.13.54

Studies on steroid monooxygenase from Cylindrocarpon radicicola ATCC 11011. Oxygenative lactonization of androstenedione to testololactone

Itagaki, E.; J. Biochem. 99, 825-832 (1986)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5-Pregnene-3beta,20alpha-diol
lactonization of androstenedione
Ilyonectria destructans
pregnenolone
and other C21-20-ketosteroids which are the substrates for oxygenative esterification of the enzyme, strongly inhibit lactonization of androstenedione
Ilyonectria destructans
progesterone
strong, competitive inhibitor of lactonization of 17-ketosteroids
Ilyonectria destructans
testosterone
lactonization of androstenedione
Ilyonectria destructans
Testosterone acetate
lactonization of androstenedione
Ilyonectria destructans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0004
-
progesterone
-
Ilyonectria destructans
0.002
-
NADPH
with androstenedione and O2 as cosubstrates
Ilyonectria destructans
0.0043
-
NADPH
with progesterone and O2 as cosubstrates
Ilyonectria destructans
0.04
-
androstenedione
-
Ilyonectria destructans
0.07
-
O2
with progesterone and NADPH as cosubstrates
Ilyonectria destructans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ilyonectria destructans
-
ATTC 11011
-
Ilyonectria destructans ATTC 11011
-
ATTC 11011
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Ilyonectria destructans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-hydroxypregnenolone + NADPH + O2
-
7389
Ilyonectria destructans
?
-
-
-
-
17alpha-hydroxypregnenolone + NADPH + O2
-
7389
Ilyonectria destructans ATTC 11011
?
-
-
-
-
17alpha-Hydroxyprogesterone + NADPH + O2
the 17-ketosteroid acts first with the enzyme, and then NADPH
7389
Ilyonectria destructans
androstenedione + acetate + NADP+ + H2O
4-androstene-3,17-dione
7389
Ilyonectria destructans
-
17alpha-Hydroxyprogesterone + NADPH + O2
the 17-ketosteroid acts first with the enzyme, and then NADPH
7389
Ilyonectria destructans ATTC 11011
androstenedione + acetate + NADP+ + H2O
4-androstene-3,17-dione
7389
Ilyonectria destructans ATTC 11011
-
androstenedione + NADPH + O2
-
7389
Ilyonectria destructans
3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
i.e. testololactone
7389
Ilyonectria destructans
-
androstenedione + NADPH + O2
-
7389
Ilyonectria destructans ATTC 11011
3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
i.e. testololactone
7389
Ilyonectria destructans ATTC 11011
-
Pregnenolone + NADPH + O2
-
7389
Ilyonectria destructans
?
-
-
-
-
Pregnenolone + NADPH + O2
-
7389
Ilyonectria destructans ATTC 11011
?
-
-
-
-
Progesterone + NADPH + O2
-
7389
Ilyonectria destructans
Testosterone acetate + NADP+ + H2O
-
-
-
-
Progesterone + NADPH + O2
-
7389
Ilyonectria destructans ATTC 11011
Testosterone acetate + NADP+ + H2O
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
progesterone monooxygenase activity
Ilyonectria destructans
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
half-life: 8 days
Ilyonectria destructans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
androstenedione monooxygenase reaction
Ilyonectria destructans
7.8
-
progesterone monooxygenase reaction
Ilyonectria destructans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
8
pH 5.5: about 20% of maximal activity, pH 8.0: about 40% of maximal activity, androstenedione monooxygenase activity
Ilyonectria destructans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5-Pregnene-3beta,20alpha-diol
lactonization of androstenedione
Ilyonectria destructans
pregnenolone
and other C21-20-ketosteroids which are the substrates for oxygenative esterification of the enzyme, strongly inhibit lactonization of androstenedione
Ilyonectria destructans
progesterone
strong, competitive inhibitor of lactonization of 17-ketosteroids
Ilyonectria destructans
testosterone
lactonization of androstenedione
Ilyonectria destructans
Testosterone acetate
lactonization of androstenedione
Ilyonectria destructans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0004
-
progesterone
-
Ilyonectria destructans
0.002
-
NADPH
with androstenedione and O2 as cosubstrates
Ilyonectria destructans
0.0043
-
NADPH
with progesterone and O2 as cosubstrates
Ilyonectria destructans
0.04
-
androstenedione
-
Ilyonectria destructans
0.07
-
O2
with progesterone and NADPH as cosubstrates
Ilyonectria destructans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Ilyonectria destructans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-hydroxypregnenolone + NADPH + O2
-
7389
Ilyonectria destructans
?
-
-
-
-
17alpha-hydroxypregnenolone + NADPH + O2
-
7389
Ilyonectria destructans ATTC 11011
?
-
-
-
-
17alpha-Hydroxyprogesterone + NADPH + O2
the 17-ketosteroid acts first with the enzyme, and then NADPH
7389
Ilyonectria destructans
androstenedione + acetate + NADP+ + H2O
4-androstene-3,17-dione
7389
Ilyonectria destructans
-
17alpha-Hydroxyprogesterone + NADPH + O2
the 17-ketosteroid acts first with the enzyme, and then NADPH
7389
Ilyonectria destructans ATTC 11011
androstenedione + acetate + NADP+ + H2O
4-androstene-3,17-dione
7389
Ilyonectria destructans ATTC 11011
-
androstenedione + NADPH + O2
-
7389
Ilyonectria destructans
3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
i.e. testololactone
7389
Ilyonectria destructans
-
androstenedione + NADPH + O2
-
7389
Ilyonectria destructans ATTC 11011
3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
i.e. testololactone
7389
Ilyonectria destructans ATTC 11011
-
Pregnenolone + NADPH + O2
-
7389
Ilyonectria destructans
?
-
-
-
-
Pregnenolone + NADPH + O2
-
7389
Ilyonectria destructans ATTC 11011
?
-
-
-
-
Progesterone + NADPH + O2
-
7389
Ilyonectria destructans
Testosterone acetate + NADP+ + H2O
-
-
-
-
Progesterone + NADPH + O2
-
7389
Ilyonectria destructans ATTC 11011
Testosterone acetate + NADP+ + H2O
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
progesterone monooxygenase activity
Ilyonectria destructans
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4
-
half-life: 8 days
Ilyonectria destructans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
androstenedione monooxygenase reaction
Ilyonectria destructans
7.8
-
progesterone monooxygenase reaction
Ilyonectria destructans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
8
pH 5.5: about 20% of maximal activity, pH 8.0: about 40% of maximal activity, androstenedione monooxygenase activity
Ilyonectria destructans
Other publictions for EC 1.14.13.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Penicillium lanosocoeruleum
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3
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1
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-
-
-
-
-
2
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-
2
-
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-
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1
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-
-
-
-
727906
Franceschini
Exploring the structural basis ...
Rhodococcus rhodochrous
J. Biol. Chem.
287
22626-22634
2012
-
-
1
1
5
-
-
5
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
6
-
-
-
2
-
-
-
-
-
1
2
1
5
-
-
-
-
5
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
6
-
-
-
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-
-
-
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11
11
693956
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Molecular evolution of a stero ...
Bacillus megaterium
Lipids
43
1133-1141
2008
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1
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3
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1
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1
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2
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-
659076
Morii
Steroid monooxygenase of Rhodo ...
Rhodococcus rhodochrous
J. Biochem.
126
624-631
1999
-
-
1
-
-
-
-
2
-
-
3
1
-
4
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
-
3
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7389
Itagaki
Studies on steroid monooxygena ...
Ilyonectria destructans, Ilyonectria destructans ATTC 11011
J. Biochem.
99
825-832
1986
-
-
-
-
-
-
5
5
-
-
-
-
-
4
-
-
-
-
-
-
1
-
10
-
1
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
-
-
-
-
-
-
-
-
1
-
10
-
1
-
1
-
2
1
-
-
-
-
-
-
-
-
7390
Itagaki
Studies on steroid monooxygena ...
Ilyonectria destructans, Ilyonectria destructans ATTC 11011
J. Biochem.
99
815-824
1986
-
-
-
-
-
-
-
3
-
-
2
-
-
4
-
-
1
-
-
1
-
1
17
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
1
-
1
17
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-