BRENDA - Enzyme Database show
show all sequences of 1.14.13.54

Molecular evolution of a steroid hydroxylating cytochrome P450 using a versatile steroid detection system for screening

Virus, C.; Bernhardt, R.; Lipids 43, 1133-1141 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Bacillus megaterium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus megaterium
-
strain ATCC 13368
-
Renatured (Commentary)
Commentary
Organism
reconstitution of recombinant protein using adrenodoxin and adrenodoxin reductase
Bacillus megaterium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-deoxycortisol + NADPH + O2
-
693956
Bacillus megaterium
? + NADP+ + H2O
-
-
-
?
Progesterone + NADPH + O2
-
693956
Bacillus megaterium
Testosterone acetate + NADP+ + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Bacillus megaterium
Renatured (Commentary) (protein specific)
Commentary
Organism
reconstitution of recombinant protein using adrenodoxin and adrenodoxin reductase
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-deoxycortisol + NADPH + O2
-
693956
Bacillus megaterium
? + NADP+ + H2O
-
-
-
?
Progesterone + NADPH + O2
-
693956
Bacillus megaterium
Testosterone acetate + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.13.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728352
Swizdor
Baeyer-Villiger oxidation of s ...
Penicillium lanosocoeruleum
Molecules
18
13812-13822
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727906
Franceschini
Exploring the structural basis ...
Rhodococcus rhodochrous
J. Biol. Chem.
287
22626-22634
2012
-
-
1
1
5
-
-
5
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
6
-
-
-
2
-
-
-
-
-
1
2
1
5
-
-
-
-
5
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
11
11
693956
Virus
Molecular evolution of a stero ...
Bacillus megaterium
Lipids
43
1133-1141
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659076
Morii
Steroid monooxygenase of Rhodo ...
Rhodococcus rhodochrous
J. Biochem.
126
624-631
1999
-
-
1
-
-
-
-
2
-
-
3
1
-
5
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
-
3
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7389
Itagaki
Studies on steroid monooxygena ...
Ilyonectria destructans, Ilyonectria destructans ATTC 11011
J. Biochem.
99
825-832
1986
-
-
-
-
-
-
5
5
-
-
-
-
-
5
-
-
-
-
-
-
1
-
10
-
1
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
5
-
-
-
-
-
-
-
-
-
-
1
-
10
-
1
-
1
-
2
1
-
-
-
-
-
-
-
-
7390
Itagaki
Studies on steroid monooxygena ...
Ilyonectria destructans, Ilyonectria destructans ATTC 11011
J. Biochem.
99
815-824
1986
-
-
-
-
-
-
-
3
-
-
2
-
-
5
-
-
1
-
-
1
-
1
17
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
2
-
-
-
-
1
-
1
-
1
17
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-