Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Rhodococcus rhodochrous |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00044 | - |
NADPH | pH 8.3, 37°C | Rhodococcus rhodochrous | |
0.055 | - |
progesterone | pH 8.3, 37°C | Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Rhodococcus rhodochrous |
60133 | - |
x * 60133, calculated from the deduced amino acid sequence | Rhodococcus rhodochrous |
60919 | - |
x * 60919, holoenzyme, calculated from the deduced amino acid sequence | Rhodococcus rhodochrous |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + NADPH + H+ + O2 | Rhodococcus rhodochrous | - |
Testosterone acetate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
starin IFO 2228 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Rhodococcus rhodochrous |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.95 | - |
purified enzyme | Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + NADPH + H+ + O2 | - |
Rhodococcus rhodochrous | Testosterone acetate + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Rhodococcus rhodochrous |
? | x * 60133, calculated from the deduced amino acid sequence | Rhodococcus rhodochrous |
? | x * 60919, holoenzyme, calculated from the deduced amino acid sequence | Rhodococcus rhodochrous |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodococcus rhodochrous | |
NADPH | - |
Rhodococcus rhodochrous |