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Literature summary for 1.14.13.50 extracted from
- Enzymatic dehalogenation of pentachlorophenol by Pseudomonas fluorescens of the microbial community from tannery effluent (2003), Curr. Microbiol., 47, 65-70.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Pseudomonas fluorescens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
gel filtration | Pseudomonas fluorescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pentachlorophenol + NADPH + H+ + O2 | Pseudomonas fluorescens | pentachlorophenol degradation | tetrachlorohydroquinone + NADP+ + chloride + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from culture extract to homogeneity, about 13fold | Pseudomonas fluorescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | isolate from contaminated site | Pseudomonas fluorescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
purified enzyme | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pentachlorophenol + NADPH + H+ + O2 | - |
Pseudomonas fluorescens | tetrachlorohydroquinone + NADP+ + chloride + H2O | - |
? | |
| pentachlorophenol + NADPH + H+ + O2 | pentachlorophenol degradation | Pseudomonas fluorescens | tetrachlorohydroquinone + NADP+ + chloride + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 24000, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PCP-4-monooxygenase | - |
Pseudomonas fluorescens |
| PCP-monooxygenase | - |
Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Pseudomonas fluorescens | |
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Release 2023.1 (January 2023)
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