Literature summary for 1.14.13.50 extracted from

Nakamura, T.; Motoyama, T.; Hirokawa, T.; Hirono, S.; Yamaguchi, I.
Computer-aided modeling of pentachlorophenol 4-monooxygenase and site-directed mutagenesis of its active site (2003), Chem. Pharm. Bull., 51, 1293-1298.

Search for more literature for 1.14.13.50

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	Sphingobium chlorophenolicum

Protein Variants

Protein Variants	Comment	Organism
F223A	site-directed mutagenesis, increased activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
F225A	site-directed mutagenesis, increased activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
F85A	site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
F87A	site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, highly reduced activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
R235E	site-directed mutagenesis, reduced activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
R235K	site-directed mutagenesis, increased activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
R235K	site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, reduced activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
Y216A	site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
Y216F	site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
Y397A	site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme	Sphingobium chlorophenolicum
Y397F	site-directed mutagenesis, 60% increased activity compared to the wild-type enzyme	Sphingobium chlorophenolicum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Sphingobium chlorophenolicum	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	x * 60000, SDS-PAGE	Sphingobium chlorophenolicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pentachlorophenol + NADPH + H+ + O2	Sphingobium chlorophenolicum	pentachlorophenol degradation	tetrachlorohydroquinone + NADP+ + chloride + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Sphingobium chlorophenolicum	-	strain ATCC 39723	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)	Sphingobium chlorophenolicum

Reaction

Reaction	Comment	Organism	Reaction ID
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O	benzene rings of Phe85, Phe87, Tyr397, as well as Tyr216 and the positive charge of Arg235 are important for activity and stabilization of the active site, reaction mechanism	Sphingobium chlorophenolicum	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pentachlorophenol + NADPH + H+ + O2	-	Sphingobium chlorophenolicum	tetrachlorohydroquinone + NADP+ + chloride + H2O	-	?
pentachlorophenol + NADPH + H+ + O2	pentachlorophenol degradation	Sphingobium chlorophenolicum	tetrachlorohydroquinone + NADP+ + chloride + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 60000, SDS-PAGE	Sphingobium chlorophenolicum
More	homology modeling and model building of three-dimensional structure	Sphingobium chlorophenolicum

Synonyms

Synonyms	Comment	Organism
PcpB	-	Sphingobium chlorophenolicum
pentachlorophenol 4-monooxygenase	-	Sphingobium chlorophenolicum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Sphingobium chlorophenolicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Sphingobium chlorophenolicum

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Sphingobium chlorophenolicum
NADPH	-	Sphingobium chlorophenolicum

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Release 2023.1 (January 2023)