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Literature summary for 1.14.13.50 extracted from

  • Lange, C.C.; Schneider, B.J.; Orser, C.S.
    Verification of the role of PCP 4-monooxygenase in chlorine elimination from pentachlorophenol by Flavobacterium sp. strain ATCC 39723 (1996), Biochem. Biophys. Res. Commun., 219, 146-149.
    View publication on PubMed
Search for more literature for 1.14.13.50

Cloned(Commentary)

Cloned (Comment) Organism
pcpB gene, expression in Escherichia coli and Flavobacterium sp. ATCC 39723 of wild-type and isogenic mutant, DNA sequence analysis Flavobacterium sp.

Protein Variants

Protein Variants Comment Organism
additional information targeted mutagenesis via allelic exchange, construction of isogenic mutant without PCP 4-monooxygenase activity and 2 cointegration mutants with slow activity Flavobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pentachlorophenol + 2 NADPH + H+ + O2 Flavobacterium sp. solitary oxygenolytic pathway for removal of the primary chlorine during PCP degradation, not a hydrolytic one tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
 ?
pentachlorophenol + 2 NADPH + H+ + O2 Flavobacterium sp. wild-type and mutant strains tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
 strain ATCC 39723
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information broad substrate and catalytic reaction range Flavobacterium sp. ?
-
 ?
pentachlorophenol + 2 NADPH + H+ + O2 solitary oxygenolytic pathway for removal of the primary chlorine during PCP degradation, not a hydrolytic one Flavobacterium sp. tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
 ?
pentachlorophenol + 2 NADPH + H+ + O2 wild-type and mutant strains Flavobacterium sp. tetrachlorohydroquinone + 2 NADP+ + Cl- + H2O
-
 ?
pentachlorophenol + NADPH + H+ + O2
-
 Flavobacterium sp. 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Flavobacterium sp.
NADPH requirement Flavobacterium sp.