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Literature summary for 1.14.13.5 extracted from

  • Watanabe, T.; Kambe, H.; Imamura, I.; Taguchi, Y.; Tamura, T.; Wada, H.
    Method for enzymatic determination of imidazole acetic acid (1983), Anal. Biochem., 130, 321-327.
    View publication on PubMed
Search for more literature for 1.14.13.5

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxyindoleacetate 11% inhibition at 4.5 mM Pseudomonas sp.
dihydroxymandelate 53% inhibition at 4.5 mM Pseudomonas sp.
Dihydroxyphenylacetate 66% inhibition at 4.5 mM Pseudomonas sp.
homogentisate 50% inhibition at 4.5 mM Pseudomonas sp.
homovanillic acid 5% inhibition at 4.5 mM Pseudomonas sp.
Imidazolepyruvate 56% inhibition at 4.5 mM Pseudomonas sp.
indoleacetate 22% inhibition at 4.5 mM Pseudomonas sp.
m-hydroxyphenylacetate 79% inhibition at 4.5 mM Pseudomonas sp.
N-methylimidazoleacetate 11% inhibition at 4.5 mM Pseudomonas sp.
p-hydroxybenzoate 38% inhibition at 4.5 mM Pseudomonas sp.
p-hydroxyphenylacetate 47% inhibition at 4.5 mM Pseudomonas sp.
phenylacetate 25% inhibition at 4.5 mM Pseudomonas sp.
vanillyl mandelic acid 9% inhibition at 4.5 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 imidazoleacetate
-
 Pseudomonas sp.
23
-
 5-hydroxyindoleacetate
-
 Pseudomonas sp.
44
-
 N-methylimidazoleacetate
-
 Pseudomonas sp.
64
-
 indoleacetate
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-imidazoleacetate + NADH + O2 Pseudomonas sp.
-
 5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using ammonium sulfate precipitation and column chromatography on DEAE-Sephadex A-50 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, stable for at least 6 months Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-imidazoleacetate + NAD(P)H + O2 + H+ highly specific for 4-imidazoleacetate Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD(P)+ + H2O
-
 ?
4-imidazoleacetate + NADH + O2
-
 Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
 ?
5-hydroxyindoleacetate + NAD(P)H + O2 + H+ 9.4% relative activity to 4-imidazoleacetate Pseudomonas sp. ? + NAD(P)+ + H2O
-
 ?
imidazolepropionate + NAD(P)H + O2 + H+ 9.5% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxyimidazolepropionate + NAD(P)+ + H2O
-
 ?
imidazolepyruvate + O2 + NAD(P)H + H+ 2.8% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxyimidazolepyruvate + NAD(P)+ + H2O
-
 ?
indoleacetate + NAD(P)H + O2 + H+ 7.3% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxyindoleacetate + NAD(P)+ + H2O
-
 ?
N-methylimidazoleacetate + NAD(P)H + O2 + H+ 7.6% relative activity to 4-imidazoleacetate Pseudomonas sp. 5-hydroxy-N-methylimidazoleacetate + NAD(P)+ + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pseudomonas sp.