Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Dihydroxybiphenyl | inhibits reaction with 2-hydroxybiphenyl | Pseudomonas nitroreducens | |
AgNO3 | 0.01 mM, complete inhibition | Pseudomonas nitroreducens | |
CuSO4 | 0.01 mM, complete inhibition | Pseudomonas nitroreducens | |
FeSO4 | 0.08 mM, 30% inhibition | Pseudomonas nitroreducens | |
HgCl2 | 0.01 mM, complete inhibition | Pseudomonas nitroreducens | |
NaCl | 10 mM, 36% inhibition. 100 mM, 89% inhibition | Pseudomonas nitroreducens | |
p-hydroxymercuribenzoate | partial protection in presence of 2-hydroxybiphenyl, reversed by excess of dithiothreitol | Pseudomonas nitroreducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
2-Hydroxybiphenyl | reaction with NADH and O2 | Pseudomonas nitroreducens | |
0.0031 | - |
2-Hydroxybiphenyl | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
0.0034 | - |
2,2'-Dihydroxybiphenyl | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
0.004 | - |
2,2'-Dihydroxybiphenyl | reaction with NADH and O2 | Pseudomonas nitroreducens | |
0.0057 | - |
2-sec-Butylphenol | reaction with NADH or NADPH and O2 | Pseudomonas nitroreducens | |
0.0216 | - |
NADH | reaction with 2,2'-dihydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
0.0268 | - |
NADH | reaction with 2-hydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
0.0292 | - |
O2 | reaction with 2-hydroxybiphenyl and NADH | Pseudomonas nitroreducens | |
0.0943 | - |
NADPH | reaction with 2,2'-dihydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
0.137 | - |
NADPH | reaction with 2-hydroxybiphenyl and O2 | Pseudomonas nitroreducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, SDS-PAGE | Pseudomonas nitroreducens |
256000 | - |
gel filtration | Pseudomonas nitroreducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybiphenyl + NADH + O2 | Pseudomonas nitroreducens | first enzyme of 2-hydroxybiphenyl degradation | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | Pseudomonas nitroreducens HBP1 | first enzyme of 2-hydroxybiphenyl degradation | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas nitroreducens | - |
HBP1 | - |
Pseudomonas nitroreducens HBP1 | - |
HBP1 | - |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme | Pseudomonas nitroreducens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas nitroreducens |
Storage Stability | Organism |
---|---|
-20°C, pure enzyme at concentration of 3.8 mg/ml in 50 mM phosphate buffer, pH 7.5, stable for at least 6 months | Pseudomonas nitroreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-dihydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens | 2,2',3-trihydroxybiphenyl + NAD+ + H2O | - |
? | |
2,2'-dihydroxybiphenyl + NADPH + O2 | - |
Pseudomonas nitroreducens | 2,2',3-trihydroxybiphenyl + NADP+ + H2O | - |
? | |
2,5-dihydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens | 2,3,5-trihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-ethylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-ethylbenzene + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | first enzyme of 2-hydroxybiphenyl degradation | Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADH + O2 | first enzyme of 2-hydroxybiphenyl degradation | Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
2-hydroxybiphenyl + NADPH + O2 | - |
Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NADP+ + H2O | - |
? | |
2-hydroxybiphenyl + NADPH + O2 | - |
Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NADP+ + H2O | - |
? | |
2-methylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-methylbenzene + NAD+ + H2O | - |
? | |
2-propylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-propylbenzene + NAD+ + H2O | - |
? | |
2-propylphenol + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 1,2-dihydroxy-3-propylbenzene + NAD+ + H2O | - |
? | |
2-sec-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 2-sec-butylcatechol + NAD+ + H2O | - |
? | |
2-sec-butylphenol + NADPH + O2 | - |
Pseudomonas nitroreducens | 2-sec-butylcatechol + NADP+ + H2O | - |
? | |
2-tert-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-tert-butylbenzene + NAD+ + H2O | - |
? | |
additional information | the substrates partially uncouple oxygen activation from hydroxylation with resultant reduction of both atoms of oxygen to form hydrogen peroxide | Pseudomonas nitroreducens | ? | - |
? | |
additional information | the substrates partially uncouple oxygen activation from hydroxylation with resultant reduction of both atoms of oxygen to form hydrogen peroxide | Pseudomonas nitroreducens HBP1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 60000, SDS-PAGE | Pseudomonas nitroreducens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9 | - |
2,2'-Dihydroxybiphenyl | reaction with NADH and O2 | Pseudomonas nitroreducens | |
9.4 | - |
2,2'-Dihydroxybiphenyl | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
9.8 | - |
NADH | reaction with 2,2'-dihydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
10.2 | - |
2-sec-Butylphenol | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
11.2 | - |
NADPH | reaction with 2,2'-dihydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
12.8 | - |
2-Hydroxybiphenyl | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
14.9 | - |
2-Hydroxybiphenyl | reaction with NADH and O2 | Pseudomonas nitroreducens | |
15.8 | - |
2-sec-Butylphenol | reaction with NADH and O2 | Pseudomonas nitroreducens | |
16.2 | - |
NADH | reaction with 2-hydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
16.2 | - |
O2 | reaction with 2-hydroxybiphenyl and NADH | Pseudomonas nitroreducens | |
18.8 | - |
NADPH | reaction with 2-hydroxybiphenyl and O2 | Pseudomonas nitroreducens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas nitroreducens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.8 | more than 80% of maximal activity at pH 7.2 and pH 7.8, beyond pH 7.8 activity declines abruptly with increasing pH | Pseudomonas nitroreducens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one molecule of noncovalently bound FAD per subunit | Pseudomonas nitroreducens | |
NADH | - |
Pseudomonas nitroreducens | |
NADPH | can replace NADH as electron donor, Km-value for NADPH is much higher than for NADH | Pseudomonas nitroreducens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
2,3-Dihydroxybiphenyl | - |
Pseudomonas nitroreducens |