Literature summary for 1.14.13.44 extracted from

Suske, W.A.; Held, M.; Schmid, A.; Fleischmann, T.; Wubbolts, M.G.; Kohler, H.P.E.
Purification and characterization of 2-hydroxybiphenyl 3-monooxygenase, a novel NADH-dependent, FAD-containing aromatic hydroxylase from Pseudomonas azelaica HBP1 (1997), J. Biol. Chem., 272, 24257-24265.

Search for more literature for 1.14.13.44

Inhibitors

Inhibitors	Comment	Organism	Structure
2,3-Dihydroxybiphenyl	inhibits reaction with 2-hydroxybiphenyl	Pseudomonas nitroreducens
AgNO3	0.01 mM, complete inhibition	Pseudomonas nitroreducens
CuSO4	0.01 mM, complete inhibition	Pseudomonas nitroreducens
FeSO4	0.08 mM, 30% inhibition	Pseudomonas nitroreducens
HgCl2	0.01 mM, complete inhibition	Pseudomonas nitroreducens
NaCl	10 mM, 36% inhibition. 100 mM, 89% inhibition	Pseudomonas nitroreducens
p-hydroxymercuribenzoate	partial protection in presence of 2-hydroxybiphenyl, reversed by excess of dithiothreitol	Pseudomonas nitroreducens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0028	-	2-Hydroxybiphenyl	reaction with NADH and O2	Pseudomonas nitroreducens
0.0031	-	2-Hydroxybiphenyl	reaction with NADPH and O2	Pseudomonas nitroreducens
0.0034	-	2,2'-Dihydroxybiphenyl	reaction with NADPH and O2	Pseudomonas nitroreducens
0.004	-	2,2'-Dihydroxybiphenyl	reaction with NADH and O2	Pseudomonas nitroreducens
0.0057	-	2-sec-Butylphenol	reaction with NADH or NADPH and O2	Pseudomonas nitroreducens
0.0216	-	NADH	reaction with 2,2'-dihydroxybiphenyl and O2	Pseudomonas nitroreducens
0.0268	-	NADH	reaction with 2-hydroxybiphenyl and O2	Pseudomonas nitroreducens
0.0292	-	O2	reaction with 2-hydroxybiphenyl and NADH	Pseudomonas nitroreducens
0.0943	-	NADPH	reaction with 2,2'-dihydroxybiphenyl and O2	Pseudomonas nitroreducens
0.137	-	NADPH	reaction with 2-hydroxybiphenyl and O2	Pseudomonas nitroreducens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	4 * 60000, SDS-PAGE	Pseudomonas nitroreducens
256000	-	gel filtration	Pseudomonas nitroreducens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-hydroxybiphenyl + NADH + O2	Pseudomonas nitroreducens	first enzyme of 2-hydroxybiphenyl degradation	2,3-dihydroxybiphenyl + NAD+ + H2O	-	?
2-hydroxybiphenyl + NADH + O2	Pseudomonas nitroreducens HBP1	first enzyme of 2-hydroxybiphenyl degradation	2,3-dihydroxybiphenyl + NAD+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas nitroreducens	-	HBP1	-
Pseudomonas nitroreducens HBP1	-	HBP1	-

Purification (Commentary)

Purification (Comment)	Organism
native and recombinant enzyme	Pseudomonas nitroreducens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Pseudomonas nitroreducens

Storage Stability

Storage Stability	Organism
-20°C, pure enzyme at concentration of 3.8 mg/ml in 50 mM phosphate buffer, pH 7.5, stable for at least 6 months	Pseudomonas nitroreducens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,2'-dihydroxybiphenyl + NADH + O2	-	Pseudomonas nitroreducens	2,2',3-trihydroxybiphenyl + NAD+ + H2O	-	?
2,2'-dihydroxybiphenyl + NADPH + O2	-	Pseudomonas nitroreducens	2,2',3-trihydroxybiphenyl + NADP+ + H2O	-	?
2,5-dihydroxybiphenyl + NADH + O2	-	Pseudomonas nitroreducens	2,3,5-trihydroxybiphenyl + NAD+ + H2O	-	?
2-ethylphenol + NADH + O2	-	Pseudomonas nitroreducens	1,2-dihydroxy-3-ethylbenzene + NAD+ + H2O	-	?
2-hydroxybiphenyl + NADH + O2	-	Pseudomonas nitroreducens	2,3-dihydroxybiphenyl + NAD+ + H2O	-	?
2-hydroxybiphenyl + NADH + O2	first enzyme of 2-hydroxybiphenyl degradation	Pseudomonas nitroreducens	2,3-dihydroxybiphenyl + NAD+ + H2O	-	?
2-hydroxybiphenyl + NADH + O2	-	Pseudomonas nitroreducens HBP1	2,3-dihydroxybiphenyl + NAD+ + H2O	-	?
2-hydroxybiphenyl + NADH + O2	first enzyme of 2-hydroxybiphenyl degradation	Pseudomonas nitroreducens HBP1	2,3-dihydroxybiphenyl + NAD+ + H2O	-	?
2-hydroxybiphenyl + NADPH + O2	-	Pseudomonas nitroreducens	2,3-dihydroxybiphenyl + NADP+ + H2O	-	?
2-hydroxybiphenyl + NADPH + O2	-	Pseudomonas nitroreducens HBP1	2,3-dihydroxybiphenyl + NADP+ + H2O	-	?
2-methylphenol + NADH + O2	-	Pseudomonas nitroreducens	1,2-dihydroxy-3-methylbenzene + NAD+ + H2O	-	?
2-propylphenol + NADH + O2	-	Pseudomonas nitroreducens	1,2-dihydroxy-3-propylbenzene + NAD+ + H2O	-	?
2-propylphenol + NADH + O2	-	Pseudomonas nitroreducens HBP1	1,2-dihydroxy-3-propylbenzene + NAD+ + H2O	-	?
2-sec-butylphenol + NADH + O2	-	Pseudomonas nitroreducens	2-sec-butylcatechol + NAD+ + H2O	-	?
2-sec-butylphenol + NADPH + O2	-	Pseudomonas nitroreducens	2-sec-butylcatechol + NADP+ + H2O	-	?
2-tert-butylphenol + NADH + O2	-	Pseudomonas nitroreducens	1,2-dihydroxy-3-tert-butylbenzene + NAD+ + H2O	-	?
additional information	the substrates partially uncouple oxygen activation from hydroxylation with resultant reduction of both atoms of oxygen to form hydrogen peroxide	Pseudomonas nitroreducens	?	-	?
additional information	the substrates partially uncouple oxygen activation from hydroxylation with resultant reduction of both atoms of oxygen to form hydrogen peroxide	Pseudomonas nitroreducens HBP1	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 60000, SDS-PAGE	Pseudomonas nitroreducens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9	-	2,2'-Dihydroxybiphenyl	reaction with NADH and O2	Pseudomonas nitroreducens
9.4	-	2,2'-Dihydroxybiphenyl	reaction with NADPH and O2	Pseudomonas nitroreducens
9.8	-	NADH	reaction with 2,2'-dihydroxybiphenyl and O2	Pseudomonas nitroreducens
10.2	-	2-sec-Butylphenol	reaction with NADPH and O2	Pseudomonas nitroreducens
11.2	-	NADPH	reaction with 2,2'-dihydroxybiphenyl and O2	Pseudomonas nitroreducens
12.8	-	2-Hydroxybiphenyl	reaction with NADPH and O2	Pseudomonas nitroreducens
14.9	-	2-Hydroxybiphenyl	reaction with NADH and O2	Pseudomonas nitroreducens
15.8	-	2-sec-Butylphenol	reaction with NADH and O2	Pseudomonas nitroreducens
16.2	-	NADH	reaction with 2-hydroxybiphenyl and O2	Pseudomonas nitroreducens
16.2	-	O2	reaction with 2-hydroxybiphenyl and NADH	Pseudomonas nitroreducens
18.8	-	NADPH	reaction with 2-hydroxybiphenyl and O2	Pseudomonas nitroreducens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Pseudomonas nitroreducens

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.2	7.8	more than 80% of maximal activity at pH 7.2 and pH 7.8, beyond pH 7.8 activity declines abruptly with increasing pH	Pseudomonas nitroreducens

Cofactor

Cofactor	Comment	Organism	Structure
FAD	one molecule of noncovalently bound FAD per subunit	Pseudomonas nitroreducens
NADH	-	Pseudomonas nitroreducens
NADPH	can replace NADH as electron donor, Km-value for NADPH is much higher than for NADH	Pseudomonas nitroreducens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.9	-	2,3-Dihydroxybiphenyl	-	Pseudomonas nitroreducens

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Release 2023.1 (January 2023)