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Literature summary for 1.14.13.43 extracted from

  • Fuji, I.; Ebizuka, Y.; Sankawa, U.
    A novel anthraquinone ring cleavage enzyme from Aspergillus terreus (1988), J. Biochem., 103, 878-883.
    View publication on PubMed

General Stability

General Stability Organism
very unstable, polyols, non-ionic detergents and EDTA have stabilizing effects Aspergillus terreus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Aspergillus terreus 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
questin + NADPH + H+ + O2 Aspergillus terreus absolute requirement for oxygen and NADPH demethylsulochrin + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus terreus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, additional functional protein or proteins required for full activity Aspergillus terreus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus terreus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.03
-
crude extract Aspergillus terreus

Storage Stability

Storage Stability Organism
cold room, 0.01 M Tris-HCl, pH 7.5, 50% ethylene glycol, v/v, 1 mM EDTA, 0.05% Tween 80, w/v, 70-80% of its original activity after 2 days Aspergillus terreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
questin + NADPH + H+ + O2 absolute requirement for oxygen and NADPH Aspergillus terreus demethylsulochrin + NADP+
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
4
-
complete loss of activity overnight Aspergillus terreus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Aspergillus terreus

Cofactor

Cofactor Comment Organism Structure
NADPH highly specific Aspergillus terreus