KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
NADH | - |
Pseudomonas sp. | |
0.47 | - |
N-ethylmaleimide | - |
Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. KB740 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of isolated and reconstituted enzyme in different fractions after purification | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-196°C stable | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-CoA + NAD(P)H + O2 | i.e. anthraniloyl-CoA | Pseudomonas sp. | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+ | - |
? | |
2-aminobenzoyl-CoA + NAD(P)H + O2 | i.e. anthraniloyl-CoA | Pseudomonas sp. KB740 | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD(P)+ | - |
? | |
2-hydroxybenzoyl-CoA + NAD(P)H + O2 | i.e. salicoyl-CoA, weak substrate, is probably monooxygenated but not hydrogenated | Pseudomonas sp. | ? | - |
? | |
2-hydroxybenzoyl-CoA + NAD(P)H + O2 | i.e. salicoyl-CoA, weak substrate, is probably monooxygenated but not hydrogenated | Pseudomonas sp. KB740 | ? | - |
? | |
anthraniloyl-CoA + NADH + H+ + O2 | - |
Pseudomonas sp. | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD+ | - |
r | |
anthraniloyl-CoA + NADH + H+ + O2 | - |
Pseudomonas sp. KB740 | 2-amino-5-oxocyclohex-1-enecarboxyl-CoA + H2O + NAD+ | - |
r | |
N-ethylmaleimide + NAD(P)H + H+ | - |
Pseudomonas sp. | N-ethylsuccinimide + NAD(P)+ | - |
? | |
N-ethylmaleimide + NAD(P)H + H+ | - |
Pseudomonas sp. KB740 | N-ethylsuccinimide + NAD(P)+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | three dimeric forms: 1. alpha,alpha, 2. alpha',alpha', 3. alpha,alpha' | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
50% loss of activity in 2 days when stored highly concentrated, in dilute solutions it loses 50% of activity in 2 min, NADH, FAD and 2-aminobenzoyl CoA enhance stability | Pseudomonas sp. |
25 | - |
50% loss of activity in 2 days when stored highly concentrated | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the three dimeric forms alphaalpha, alpha'alpha' and alphaalpha' differ in their mode of binding FAD | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. |