Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MeHg | methylmercury, impact of chronic MeHg intoxication on NADPH diaphorase (NADPH-d) activity and astrocyte mobilization in the visual cortex of the rat, overview. MeHg accumulates in the central nervous system (CNS) and preferentially accumulates in astrocytes, and it causes several neurotoxic effects, such as visual-field constriction, cerebellar ataxia and multimodal sensory disorders. MeHg-intoxicated animals display a significant decrease of NADPH-d neuropil reactivity across the visual cortex when compared to controls. The decreased neuropil reactivity to NADPH-d may also be related to MeHg-induced astrocytic dysfunction | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Rattus norvegicus | overall reaction | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
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2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Rattus norvegicus Wistar | overall reaction | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P29476 | - |
- |
Rattus norvegicus Wistar | P29476 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
astrocyte | - |
Rattus norvegicus | - |
brain | visual cortex, use of the NADPH-d histochemistry technique to evaluate the expression of NOS across the nervous system | Rattus norvegicus | - |
additional information | pattern of NADPH-d neuropil staining and pattern of astrocyte reactivity, qualitative and quantitative analysis, overview. MeHg-intoxicated animals display a significant decrease of NADPH-d neuropil reactivity across the visual cortex when compared to controls | Rattus norvegicus | - |
neuron | the NADPH-d histochemistry stains a selective population of neurons in the brain. The staining allows the visualization of the dendritic tree, resembling a Golgi impregnation, and the cells are distributed sparsely in the cortical tissue, allowing their unbiased identification and reconstruction, quantitative analysis, overview | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | overall reaction | Rattus norvegicus | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | overall reaction | Rattus norvegicus Wistar | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
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Synonyms | Comment | Organism |
---|---|---|
NADPH diaphorase | - |
Rattus norvegicus |
NADPH-d | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | provided from the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH diaphorase) activity. In the brain, NADPH diaphorase (NADPH-d) and nNOS are strictly co-localized | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | nitric oxide (NO) is a ubiquitous gaseous cellular messenger with multiple physiological roles in the brain, such as synaptic plasticity, neurotransmission, neuronal communication, neurogenesis, learning, and memory. NO can be generated by three different isoforms of the enzyme nitric oxide synthase (NOS): neuronal NOS (nNOS), endothelial NOS (eNOS), and inducible NOS (iNOS), from L-arginine as the substrate and molecular oxygen and NADPH as cosubstrates | Rattus norvegicus |