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Literature summary for 1.14.13.39 extracted from

  • Hristov, M.; Landzhov, B.; Yakimova, K.
    Increased NADPH-diaphorase reactivity in the hypothalamic paraventricular nucleus and tanycytes following systemic administration of leptin in rats (2019), Acta Histochem., 121, 690-694 .
    View publication on PubMed

Application

Application Comment Organism
analysis by using enzyme NADPH-d histochemistry, a marker for NOS activity, the effect of leptin on NO-producing cells in the arcuate nucleus (ARC) and paraventricular nucleus (PVN) of male Wistar rats is assessed Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + 3 NADPH + 3 H+ + 4 O2 Rattus norvegicus overall reaction 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
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?
L-arginine + 3 NADPH + 3 H+ + 4 O2 Rattus norvegicus Wistar overall reaction 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
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?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P29476
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Rattus norvegicus Wistar P29476
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Source Tissue

Source Tissue Comment Organism Textmining
brain hypothalamic paraventricular nucleus and tanycytes Rattus norvegicus
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hypothalamus neurons of several nuclei of the hypothalamus, such as the arcuate nucleus (ARC) and paraventricular nucleus (PVN) Rattus norvegicus
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additional information distribution of NADPH-d-positive neurons and fibers in the paraventricular nucleus (PVN) after saline and leptin treatment, in situ overview Rattus norvegicus
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neuron neurons of several nuclei of the hypothalamus, such as the arcuate nucleus (ARC) and paraventricular nucleus (PVN) Rattus norvegicus
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paraventricular nucleus
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Rattus norvegicus
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tanycyte
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Rattus norvegicus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + 3 NADPH + 3 H+ + 4 O2 overall reaction Rattus norvegicus 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
?
L-arginine + 3 NADPH + 3 H+ + 4 O2 overall reaction Rattus norvegicus Wistar 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
?

Synonyms

Synonyms Comment Organism
NADPH-d
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Rattus norvegicus
NADPH-diaphorase
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Rattus norvegicus
nicotinamide adenine dinucleotide phosphate-diaphorase
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Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Rattus norvegicus

General Information

General Information Comment Organism
physiological function leptin-induced NO production in tanycytes may affect the neurogenesis occurs in the hypothalamus. The increased NADPH-d staining in both the arcuate nucleus (ARC) and paraventricular nucleus (PVN) of the leptin-treated rats suggests that both the PVN and ARC may be important centers in the hypothalamus for the leptin action, mediated at least in part by increased NO synthesis. The present observations also suggest that leptin may activate NOS thereby resulting in increased production of NO in hypothalamic tanycytes, possibly affecting the release of neurohormones and hypothalamic neurogenesis Rattus norvegicus