Application | Comment | Organism |
---|---|---|
analysis | by using enzyme NADPH-d histochemistry, a marker for NOS activity, the effect of leptin on NO-producing cells in the arcuate nucleus (ARC) and paraventricular nucleus (PVN) of male Wistar rats is assessed | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + 3 NADPH + 3 H+ + 4 O2 | Rattus norvegicus | overall reaction | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
L-arginine + 3 NADPH + 3 H+ + 4 O2 | Rattus norvegicus Wistar | overall reaction | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P29476 | - |
- |
Rattus norvegicus Wistar | P29476 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | hypothalamic paraventricular nucleus and tanycytes | Rattus norvegicus | - |
hypothalamus | neurons of several nuclei of the hypothalamus, such as the arcuate nucleus (ARC) and paraventricular nucleus (PVN) | Rattus norvegicus | - |
additional information | distribution of NADPH-d-positive neurons and fibers in the paraventricular nucleus (PVN) after saline and leptin treatment, in situ overview | Rattus norvegicus | - |
neuron | neurons of several nuclei of the hypothalamus, such as the arcuate nucleus (ARC) and paraventricular nucleus (PVN) | Rattus norvegicus | - |
paraventricular nucleus | - |
Rattus norvegicus | - |
tanycyte | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + 3 NADPH + 3 H+ + 4 O2 | overall reaction | Rattus norvegicus | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
L-arginine + 3 NADPH + 3 H+ + 4 O2 | overall reaction | Rattus norvegicus Wistar | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH-d | - |
Rattus norvegicus |
NADPH-diaphorase | - |
Rattus norvegicus |
nicotinamide adenine dinucleotide phosphate-diaphorase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | leptin-induced NO production in tanycytes may affect the neurogenesis occurs in the hypothalamus. The increased NADPH-d staining in both the arcuate nucleus (ARC) and paraventricular nucleus (PVN) of the leptin-treated rats suggests that both the PVN and ARC may be important centers in the hypothalamus for the leptin action, mediated at least in part by increased NO synthesis. The present observations also suggest that leptin may activate NOS thereby resulting in increased production of NO in hypothalamic tanycytes, possibly affecting the release of neurohormones and hypothalamic neurogenesis | Rattus norvegicus |