Cloned (Comment) | Organism |
---|---|
phylogenetic tree, expression of a His-tagged construct, dNOSr, comprising the dNOS reductase domain and its adjacent calmodulin binding site in Escherichia coli strain BL21 | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of flavin reduction of the recombinant enzyme, overview | Drosophila melanogaster | |
0.0032 | - |
NADPH | CaM-bound recombinant eNOS | Drosophila melanogaster | |
0.0041 | - |
NADPH | CaM-free recombinant eNOS | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Drosophila melanogaster | |
Fe2+ | required | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Drosophila melanogaster | regulatory mechanism, overview | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
additional information | Drosophila melanogaster | dNOS participates in essential developmental and behavioral aspects of the fruit fly | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged construct dNOSr from Escherichia coli strain BL21 by nickel affinity and calmodulin affinity chromatography | Drosophila melanogaster |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
steady-state catalytic activities of dNOSr | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | regulatory mechanism, overview | Drosophila melanogaster | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | conserved residue Arg1329 of nNOS enables bound NADPH to stabilize the FMN-shielded conformation, overview | Drosophila melanogaster | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
additional information | dNOS participates in essential developmental and behavioral aspects of the fruit fly | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNOS | - |
Drosophila melanogaster |
nitric-oxide synthase | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
anaerobic pre-steady-state cytochrome c reduction assay at | Drosophila melanogaster |
25 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | - |
Drosophila melanogaster | |
cytochrome c | - |
Drosophila melanogaster | |
FAD | - |
Drosophila melanogaster | |
FMN | regulation of the FMN module conformational equilibrium, overview | Drosophila melanogaster | |
NADPH | dependent on | Drosophila melanogaster |