Literature summary for 1.14.13.39 extracted from

Hong, I.S.; Kim, Y.K.; Choi, W.S.; Seo, D.W.; Yoon, J.W.; Han, J.W.; Lee, H.Y.; Lee, H.W.
Purification and characterization of nitric oxide synthase from Staphylococcus aureus (2003), FEMS Microbiol. Lett., 222, 177-182.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Calmodulin	required	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
aminoguanidine	0.01 mM, about 40% residual activity	Staphylococcus aureus
methylisothiourea	0.01 mM, about 80% residual activity	Staphylococcus aureus
N-monomethyl-L-arginine	0.01 mM, about 55% residual activity	Staphylococcus aureus
N-nitro-L-arginine methyl ester	0.01 mM, about 40% residual activity	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0134	-	L-arginine	pH 6.5, 47.5°C	Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	required	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64000	-	1 * 67000 + 1 * 64000, SDS-PAGE	Staphylococcus aureus
67000	-	1 * 67000 + 1 * 64000, SDS-PAGE	Staphylococcus aureus
135000	-	gel filtration	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	induced by methanol	-

Purification (Commentary)

Purification (Comment)	Organism
-	Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0353	-	pH 6.5, 47.5°C	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+	-	Staphylococcus aureus	2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O	-	?

Subunits

Subunits	Comment	Organism
heterodimer	1 * 67000 + 1 * 64000, SDS-PAGE	Staphylococcus aureus

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Release 2023.1 (January 2023)