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Literature summary for 1.14.13.39 extracted from

  • Wang, Z.Q.; Wei, C.C.; Santolini, J.; Panda, K.; Wang, Q.; Stuehr, D.J.
    A tryptophan that modulates tetrahydrobiopterin-dependent electron transfer in nitric oxide synthase regulates enzyme catalysis by additional mechanisms (2005), Biochemistry, 44, 4676-4690.
    View publication on PubMed
Search for more literature for 1.14.13.39

Protein Variants

Protein Variants Comment Organism
W457A increase in Km-value of tetrahydrobiopterin and L-arginine, decrease in NO synthesis activity, slower building of enzyme heme-NO complex and slower reactivity of heme-dioxy intermediate Mus musculus
W678A increase in Km-value of tetrahydrobiopterin, decrease in NO synthesis activity, slower building of enzyme heme-NO complex and slower reactivity of heme-dioxy intermediate Rattus norvegicus
W678F increase in Km-value of tetrahydrobiopterin, decrease in NO synthesis activity, slower building of enzyme heme-NO complex and slower reactivity of heme-dioxy intermediate Rattus norvegicus

General Stability

General Stability Organism
mutants less stable than wild-type, precipitation during incubation at 15°C, partial stabilization with NaCl, stabilization by purification in presence of L-arginie and tetrahydrobiopterin Rattus norvegicus
mutants less stable than wild-type, precipitation during incubation at 15°C, partial stabilization with NaCl, stabilization by purification in presence of L-arginine and tetrahydrobiopterin Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 tetrahydrobiopterin 22°C, wild type Rattus norvegicus
0.0016
-
 tetrahydrobiopterin 22°C, wild type Mus musculus
0.0022
-
 L-arginine 22°C, wild type Rattus norvegicus
0.0023
-
 L-arginine 22°C, wild type Mus musculus
0.0024
-
 L-arginine 22°C, mutant W678F Rattus norvegicus
0.0025
-
 L-arginine 22°C, mutant W678A Rattus norvegicus
0.025
-
 tetrahydrobiopterin 22°C, mutant W678A Rattus norvegicus
0.0275
-
 L-arginine 22°C, mutant W457A Mus musculus
0.1
-
 tetrahydrobiopterin 22°C, mutant W457A Mus musculus
0.114
-
 tetrahydrobiopterin 22°C, mutant W678A Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron low-spin heme iron, L-arginine and tetrahydrobiopterin bind and stabilize heme iron in five-coordinate high-spin state Rattus norvegicus
Iron low-spin heme iron, L-arginine aud tetrahydrobiopterin bind and stabilize heme iron in five-coordinate high-spin state Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P29477
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein
-
 Rattus norvegicus
flavoprotein
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
neuron
-
 Rattus norvegicus
-
neuron
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + NADPH + O2 + tetrahydrobiopterin
-
 Rattus norvegicus citrulline + NO + NADP+ + ?
-
 ?
L-arginine + NADPH + O2 + tetrahydrobiopterin
-
 Mus musculus citrulline + NO + NADP+ + ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
(6R)-tetrahydrobiopterin
-
 Rattus norvegicus
(6R)-tetrahydrobiopterin
-
 Mus musculus
heme
-
 Rattus norvegicus
heme
-
 Mus musculus
NADPH
-
 Rattus norvegicus
NADPH
-
 Mus musculus