Protein Variants | Comment | Organism |
---|---|---|
W457A | increase in Km-value of tetrahydrobiopterin and L-arginine, decrease in NO synthesis activity, slower building of enzyme heme-NO complex and slower reactivity of heme-dioxy intermediate | Mus musculus |
W678A | increase in Km-value of tetrahydrobiopterin, decrease in NO synthesis activity, slower building of enzyme heme-NO complex and slower reactivity of heme-dioxy intermediate | Rattus norvegicus |
W678F | increase in Km-value of tetrahydrobiopterin, decrease in NO synthesis activity, slower building of enzyme heme-NO complex and slower reactivity of heme-dioxy intermediate | Rattus norvegicus |
General Stability | Organism |
---|---|
mutants less stable than wild-type, precipitation during incubation at 15°C, partial stabilization with NaCl, stabilization by purification in presence of L-arginie and tetrahydrobiopterin | Rattus norvegicus |
mutants less stable than wild-type, precipitation during incubation at 15°C, partial stabilization with NaCl, stabilization by purification in presence of L-arginine and tetrahydrobiopterin | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
tetrahydrobiopterin | 22°C, wild type | Rattus norvegicus | |
0.0016 | - |
tetrahydrobiopterin | 22°C, wild type | Mus musculus | |
0.0022 | - |
L-arginine | 22°C, wild type | Rattus norvegicus | |
0.0023 | - |
L-arginine | 22°C, wild type | Mus musculus | |
0.0024 | - |
L-arginine | 22°C, mutant W678F | Rattus norvegicus | |
0.0025 | - |
L-arginine | 22°C, mutant W678A | Rattus norvegicus | |
0.025 | - |
tetrahydrobiopterin | 22°C, mutant W678A | Rattus norvegicus | |
0.0275 | - |
L-arginine | 22°C, mutant W457A | Mus musculus | |
0.1 | - |
tetrahydrobiopterin | 22°C, mutant W457A | Mus musculus | |
0.114 | - |
tetrahydrobiopterin | 22°C, mutant W678A | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | low-spin heme iron, L-arginine and tetrahydrobiopterin bind and stabilize heme iron in five-coordinate high-spin state | Rattus norvegicus | |
Iron | low-spin heme iron, L-arginine aud tetrahydrobiopterin bind and stabilize heme iron in five-coordinate high-spin state | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P29477 | - |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
flavoprotein | - |
Rattus norvegicus |
flavoprotein | - |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neuron | - |
Rattus norvegicus | - |
neuron | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + NADPH + O2 + tetrahydrobiopterin | - |
Rattus norvegicus | citrulline + NO + NADP+ + ? | - |
? | |
L-arginine + NADPH + O2 + tetrahydrobiopterin | - |
Mus musculus | citrulline + NO + NADP+ + ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
(6R)-tetrahydrobiopterin | - |
Rattus norvegicus | |
(6R)-tetrahydrobiopterin | - |
Mus musculus | |
heme | - |
Rattus norvegicus | |
heme | - |
Mus musculus | |
NADPH | - |
Rattus norvegicus | |
NADPH | - |
Mus musculus |