Cloned (Comment) | Organism |
---|---|
neuronal wild-type and natural mutant, specifically expressed in the central nervous system, expression in Escherichia coli and in insect cells of Spodoptera frugiperda via baculovirus infection | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
x-ray crystal structure of the heme-binding domains of neuronal wild-type and mutant with deletion in the heme-binding domain | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | naturally occuring neuronal mutant with a 105-amino acid deletion in the heme-binding domain as a result of in-frame mutation by specific alternative splicing, contains heme, but shows no L-arginine and NADPH-dependent citrulline-forming activity in presence of Ca2+-promoted calmodulin | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | naturally occuring neuronal mutant with a 105-amino acid deletion in the heme-binding domain as a result of in-frame mutation by specific alternative splicing, contains heme, but shows no L-arginine and NADPH-dependent citrulline-forming activity in presence of Ca2+-promoted calmodulin, the heme coordination geometry is highly abnormal | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and natural mutant, recombinant from Escherichia coli and insect cells | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | alignment of heme-binding domain amino acid sequences of NOS | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neuron | central nervous system, natural variant with 105-amino acid deletion in the heme-binding domain | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus |