Literature summary for 1.14.13.39 extracted from

Leber, A.; Hemmens, B.; Klosch, B.; Goessler, W.; Raber, G.; Mayer, B.; Schmidt, K.
Characterization of recombinant human endothelial nitric-oxide synthase purified from the yeast Pichia pastoris (1999), J. Biol. Chem., 274, 37658-37664.

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Cloned(Commentary)

Cloned (Comment)	Organism
endothelial enzyme expressed in Pichia pastoris using a highly inducible alcohol oxidase promoter PAOX1	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0039	-	L-arginine	recombinant from Pichia pastoris	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	0.8 mol per mol of subunit	Homo sapiens
Iron	heme-iron	Homo sapiens
Zinc	0.43 mol per mol of subunit	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Pichia pastoris	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
endothelium	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.19	-	purified recombinant enzyme	Homo sapiens
0.34	0.35	purified enzyme, recombinant, determined as NADPH-oxidase activity	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+	-	Homo sapiens	2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O	-	?
oxidized cytochrome c + NADPH + O2	-	Homo sapiens	reduced cytochrome c + NADP+ + H2O	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 131000-133000, recombinant, SDS-PAGE	Homo sapiens
More	gel filtration: native protein is a dimer, especially in presence of tetrahydrobiopterin and L-arginine, but dissociates at low temperature to monomers during gel electrophoresis	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
5,6,7,8-tetrahydro-L-biopterin	absolute requirement, recombinant from Pichia pastoris	Homo sapiens
Calmodulin	dependent on, endothelial enzyme	Homo sapiens
FAD	0.56 mol per mol of recombinant enzyme	Homo sapiens
FMN	0.79 mol per mol of recombinant enzyme	Homo sapiens
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)