Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid | i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme | Bos taurus | |
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid | i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme | Mus musculus | |
Ngamma-monomethyl-L-arginine | - |
Bos taurus | |
Ngamma-monomethyl-L-arginine | - |
Mus musculus | |
Ngamma-nitro-L-arginine | - |
Bos taurus | |
Ngamma-nitro-L-arginine | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Bos taurus | 5829 | - |
membrane | constitutive endothelial enzyme: predominantly membrane-bound, inducible macrophage enzyme: equally distributed between cytosol and membrane, small constitutive membrane-bound portion in murine macrophages | Mus musculus | 16020 | - |
membrane | constitutive endothelial enzyme: predominantly membrane-bound, inducible macrophage enzyme: equally distributed between cytosol and membrane, small constitutive membrane-bound portion in murine macrophages | Bos taurus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | insoluble enzyme | Mus musculus | |
Ca2+ | insoluble enzyme | Bos taurus | |
Ca2+ | constitutive endothelial and inducible membrane bound macrophage enzyme are strictly Ca2+-dependent | Mus musculus | |
Ca2+ | constitutive endothelial and inducible membrane bound macrophage enzyme are strictly Ca2+-dependent | Bos taurus | |
Ca2+ | macrophage enzyme | Mus musculus | |
Ca2+ | macrophage enzyme | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
3 isoforms exist in bovine aortic endothelial cells differentiated by substrate specificity and Ca2+-dependence | - |
Mus musculus | - |
3 isoforms exist in mouse macrophages differentiated by substrate specificity and Ca2+-dependence | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endothelium | aorta, cell culture | Mus musculus | - |
endothelium | aorta, cell culture | Bos taurus | - |
macrophage | - |
Mus musculus | - |
macrophage | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Bos taurus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
L-Ala-L-Arg + NADPH + O2 | endothelial microsomes, macrophage | Mus musculus | ? | - |
? | |
L-Ala-L-Arg + NADPH + O2 | endothelial microsomes, macrophage | Bos taurus | ? | - |
? | |
L-Arg-L-Arg + NADPH + O2 | endothelial, microsomes, macrophage | Mus musculus | ? | - |
? | |
L-Arg-L-Arg + NADPH + O2 | endothelial, microsomes, macrophage | Bos taurus | ? | - |
? | |
L-Arg-L-Arg-L-Arg + NADPH + O2 | endothelial microsomes | Mus musculus | ? | - |
? | |
L-Arg-L-Arg-L-Arg + NADPH + O2 | endothelial microsomes | Bos taurus | ? | - |
? | |
L-Arg-L-Phe + NADPH + O2 | - |
Mus musculus | ? | - |
? | |
L-Arg-L-Phe + NADPH + O2 | - |
Bos taurus | ? | - |
? | |
L-homoarginine + NADPH + O2 | constitutive endothelial membrane-bound and inducible soluble macrophage enzyme | Mus musculus | ? | - |
? | |
L-homoarginine + NADPH + O2 | constitutive endothelial membrane-bound and inducible soluble macrophage enzyme | Bos taurus | ? | - |
? | |
Ngamma-hydroxy-L-arginine + NADPH + O2 | best substrate | Mus musculus | citrulline + NADP+ + NO | - |
? | |
Ngamma-hydroxy-L-arginine + NADPH + O2 | best substrate | Bos taurus | citrulline + NADP+ + NO | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | NADPH-dependent dioxygenase | Mus musculus | |
NADPH | NADPH-dependent dioxygenase | Bos taurus |