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Literature summary for 1.14.13.39 extracted from

  • Stuehr, D.J.; Cho, H.J.; Kwon, N.S.; Weise, M.F.; Nathan, C.F.
    Purification and characterization of the cytokine-induced macrophage nitric oxide synthase: an FAD- and FMN-containing flavoprotein (1991), Proc. Natl. Acad. Sci. USA, 88, 7773-7777.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
interferon gamma activates Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
EDTA no inhibition Mus musculus
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme Mus musculus
additional information the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0003
-
NADPH
-
Mus musculus
0.002 0.0028 L-arginine
-
Mus musculus
0.0028
-
L-arginine
-
Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
dissociates irreversibly into subunits in the absence of L-arginine, FAD and tetrahydrobiopterin Mus musculus
130000
-
2 * 130000, SDS-PAGE Mus musculus
250000
-
gel filtration Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ Mus musculus enzyme of mammalian immune, cardiovascular and neural systems, synthesizing the free radical nitric oxide or a NO-releasing product 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
macrophage Ca2+-independent form Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.06
-
purified enzyme Mus musculus

Storage Stability

Storage Stability Organism
-80°C, diluted preparation stable overnight Mus musculus
4°C, 6 h stable Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
?
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ enzyme of mammalian immune, cardiovascular and neural systems, synthesizing the free radical nitric oxide or a NO-releasing product Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
?

Subunits

Subunits Comment Organism
dimer 2 * 130000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
t1/2: about 6 h Mus musculus

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin
-
Mus musculus
Calmodulin murine macrophage enzyme is Ca2+/calmodulin independent Mus musculus
FAD 2.2 mol FAD per mol of enzyme dimer Mus musculus
FAD non-covalently bound FAD Mus musculus
FMN 1.1 mol FMN per mol enzyme dimer Mus musculus
NADPH
-
Mus musculus