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Literature summary for 1.14.13.39 extracted from

  • Klatt, P.; Heinzel, B.; Mayer, B.; Ambach, E.; Werner-Felmayer, G.; Wachter, H.; Werner, E.R.
    Stimulation of human nitric oxide synthase by tetrahydrobiopterin and selective binding of the cofactor (1992), FEBS Lett., 305, 160-162.
    View publication on PubMed
Search for more literature for 1.14.13.39

Activating Compound

Activating Compound Comment Organism Structure
additional information neopterin derivatives are completely inactive and do not bind to the enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 1 * 160000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ Homo sapiens a cytokine-inducible, calcium independent and a constitutive, calcium dependent form 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 2',5'-ADP-agarose affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain Ca2+-dependent isoform Homo sapiens
-
cerebellum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.181
-
 partially purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Homo sapiens 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ a cytokine-inducible, calcium independent and a constitutive, calcium dependent form Homo sapiens 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 160000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin stimulates 4fold at 0.001 mM Homo sapiens
NADPH
-
 Homo sapiens