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Literature summary for 1.14.13.39 extracted from

  • Bredt, D.S.; Snyder, S.H.
    Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme (1990), Proc. Natl. Acad. Sci. USA, 87, 682-685.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
bovine serum albumin stabilizes Rattus norvegicus
glycerol stabilizes Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
N-(4-aminobutyl)-5-chloro-2-naphthalene sulfonamide
-
Rattus norvegicus
N-(6-Aminohexyl)-1-naphthalene sulfonamide
-
Rattus norvegicus
Trifluoperazine
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002 0.0028 L-arginine
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
1 * 150000, SDS-PAGE Rattus norvegicus
200000
-
gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2',5'-ADP-affinity and anion exchange chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.94 0.96
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
0°C, crude, t1/2: 2 days, purified t1/2: 2 h, with 1 mg/ml bovine serum albumin and 20% v/v glycerol as stabilizing agents, t1/2: 7 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
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Subunits

Subunits Comment Organism
monomer 1 * 150000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
Calmodulin activation, potent stimulator of purified, not crude, enzyme preparation Rattus norvegicus
NADPH
-
Rattus norvegicus