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Literature summary for 1.14.13.35 extracted from
- Anthranilate hydroxylase from Aspergillus niger: new type of NADPH-linked nonheme iron monooxygenase (1984), J. Bacteriol., 160, 651-655.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 50% inhibition at 0.25 mM, 80% inhibition at 0.5 mM and 95% inhibition at 1 mM | Aspergillus niger |  |
| 2,2'-dipyridyl | 50% inhibition at 0.5 mM, 80% inhibition at 1 mM | Aspergillus niger | |
| 8-hydroxyquinoline | 30% inhibition at 0.5 mM, 40% inhibition at 1 mM | Aspergillus niger | |
| additional information | not: atebrin, aminopterin | Aspergillus niger |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains 2 gatom of non-heme iron per mol | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
2 * 42000, SDS-PAGE | Aspergillus niger |
| 89000 | - |
gel filtration | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using protamine sulfate treatment, DEAE-cellulose treatment, ammonium sulfate precipitation, fractionation on Biogel P-100 column, successive negative adsorption on alumina-gel, tricalcium phosphate gel and DEAE-cellulose column, and positive adsorption on a DEAE-Sephadex A-50 column | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.193 | - |
- |
Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, ammonium sulfate precipitate, stable for at least 3 months | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| anthranilate + NADPH + O2 | - |
Aspergillus niger | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42000, SDS-PAGE | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
half-life: 14 h | Aspergillus niger |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein | Aspergillus niger | |
| NADPH | - |
Aspergillus niger | |
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Release 2023.1 (January 2023)
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