BRENDA - Enzyme Database show
show all sequences of 1.14.13.27

A new UV method for serum gamma-glutamyltransferase assay using recombinant 4-aminobenzoate hydroxylase as a coupling enzyme

Mizutani, Y.; Narikawa, T.; Satoh, T.; Sakurai, N.; Kaji, H.; Yamada, S.; Samejima, T.; J. Biochem. 126, 347-353 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
fusion protein with glutathione-S-transferase
Agaricus bisporus
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
amphoteric and anionic detergents cause inactivation
Agaricus bisporus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1117
-
4-Aminobenzoate
at pH 7
Agaricus bisporus
0.4739
-
4-Aminobenzoate
at pH 8
Agaricus bisporus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agaricus bisporus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Agaricus bisporus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.91
-
-
Agaricus bisporus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-4-aminobenzoate + NAD(P)H + O2
-
438912
Agaricus bisporus
2-chloro-4-hydroxyaniline + NAD(P)H + O2
-
438912
Agaricus bisporus
?
4-aminobenzoate + NAD(P)H + O2
-
438912
Agaricus bisporus
4-hydroxyaniline + NAD(P)+ + H2O + CO2
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Agaricus bisporus
Cloned(Commentary) (protein specific)
Commentary
Organism
fusion protein with glutathione-S-transferase
Agaricus bisporus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
amphoteric and anionic detergents cause inactivation
Agaricus bisporus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1117
-
4-Aminobenzoate
at pH 7
Agaricus bisporus
0.4739
-
4-Aminobenzoate
at pH 8
Agaricus bisporus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Agaricus bisporus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.91
-
-
Agaricus bisporus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chloro-4-aminobenzoate + NAD(P)H + O2
-
438912
Agaricus bisporus
2-chloro-4-hydroxyaniline + NAD(P)H + O2
-
438912
Agaricus bisporus
?
4-aminobenzoate + NAD(P)H + O2
-
438912
Agaricus bisporus
4-hydroxyaniline + NAD(P)+ + H2O + CO2
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Agaricus bisporus
Other publictions for EC 1.14.13.27
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
438912
Mizutani
A new UV method for serum gamm ...
Agaricus bisporus
J. Biochem.
126
347-353
1999
-
-
1
-
-
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1
2
-
-
-
-
-
3
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
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-
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-
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1
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1
-
2
-
-
-
-
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1
-
-
1
-
2
-
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-
-
1
-
-
-
-
-
-
-
-
-
438913
Tsuji
Epitope mapping of monoclonal ...
Agaricus bisporus
Biochim. Biophys. Acta
1425
628-631
1998
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
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1
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438914
Tsuji
Cloning and sequencing of cDNA ...
Agaricus bisporus
Biochim. Biophys. Acta
1309
31-36
1996
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
438910
Tsuji
-
Stereospecificity of NAD(P)H o ...
Agaricus bisporus
Biochim. Biophys. Acta
991
380-382
1989
-
-
-
-
-
-
-
-
-
-
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1
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1
1
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1
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1
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1
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1
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1
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-
438909
Tsuji
Purification and properties of ...
Agaricus bisporus
J. Biol. Chem.
261
13203-13209
1986
-
-
-
-
-
-
10
4
-
-
2
-
-
2
-
-
1
-
-
-
-
1
8
1
1
-
5
-
2
-
1
3
-
-
-
-
-
-
3
-
-
-
-
10
-
4
-
-
2
-
-
-
-
1
-
-
-
1
8
1
1
-
5
-
2
-
1
-
-
-
-
-
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-
438911
Tsuji
A unique enzyme catalyzing the ...
Agaricus bisporus
Biochem. Biophys. Res. Commun.
130
633-639
1985
-
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-
-
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4
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1
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1
-
-
1
1
1
7
-
-
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-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
4
-
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-
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-
1
-
1
1
1
7
-
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-
1
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