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Literature summary for 1.14.13.25 extracted from

  • Lettau, E.; Zill, D.; Spaeth, M.; Lorent, C.; Singh, P.K.; Lauterbach, L.
    Catalytic and spectroscopic properties of the halotolerant soluble methane monooxygenase reductase from Methylomonas methanica MC09 (2022), ChemBioChem, 23, e202100592 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0116
-
NADH pH 7.2, 36°C Methylomonas methanica

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl as a true halotolerant enzyme, MmoC still shows 50% of its specific activity at 2 M NaCl. Evaluation of salt tolerance for NADH-mediated reduction of benzyl viologen by MmoC at non-optima conditions, 23°C and pH 7.0 Methylomonas methanica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + NADH + H+ + O2 Methylomonas methanica
-
methanol + NAD+ + H2O
-
?
methane + NADH + H+ + O2 Methylomonas methanica MC09
-
methanol + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Methylomonas methanica
-
-
-
Methylomonas methanica MC09
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NADH + H+ + O2
-
Methylomonas methanica methanol + NAD+ + H2O
-
?
methane + NADH + H+ + O2
-
Methylomonas methanica MC09 methanol + NAD+ + H2O
-
?
additional information the soluble methane monooxygenase receives electrons from NADH via its reductase MmoC for oxidation of methane. The NADH-dependent reductase MmoC produces only trace amounts of superoxide, but mainly hydrogen peroxide during uncoupled turnover reactions Methylomonas methanica ?
-
-
additional information the soluble methane monooxygenase receives electrons from NADH via its reductase MmoC for oxidation of methane. The NADH-dependent reductase MmoC produces only trace amounts of superoxide, but mainly hydrogen peroxide during uncoupled turnover reactions Methylomonas methanica MC09 ?
-
-

Subunits

Subunits Comment Organism
? x * 37900, SDS-PAGE Methylomonas methanica

Synonyms

Synonyms Comment Organism
MmMmoC
-
Methylomonas methanica
MmoC
-
Methylomonas methanica
sMMO
-
Methylomonas methanica
soluble methane monooxygenase
-
Methylomonas methanica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
36
-
-
Methylomonas methanica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information the MmoC-related turnover rate or the NADH:BV oxidoreductase activity is 4585/min with an apparent molecular weight of 37.9 kDa. The hydrogen peroxide production activity of MmMmoC is 0.14 U/mg, the turnover rate of 5.2/min Methylomonas methanica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Methylomonas methanica

Cofactor

Cofactor Comment Organism Structure
FAD the specific activity of MmoC is increased significantly by 20% in presence of 25 microM FAD, whereas addition of FMN does not improve activity confirming FAD as the prosthetic group Methylomonas methanica
additional information presence of a FAD and a [2Fe2S] cluster as its prosthetic group at the reductase MmoC component of the soluble methane monooxygenase complex. The MmMmoC prefers NADH instead of NADPH. No NADPH oxidation activity can be determined at 1 mM Methylomonas methanica
NADH specific for Methylomonas methanica

General Information

General Information Comment Organism
additional information MmoC homology modeling using structure PDB ID 1KRH.1 and the crystal structure of the monomeric MMOH-MmoB complex from Methylococcus capsulatus (PDB ID 4GAM) as a templates Methylomonas methanica