Activating Compound | Comment | Organism | Structure |
---|---|---|---|
regulatory protein MMOB | the native regulatory protein MMOB is required for maximum enzyme activity | Methylococcus capsulatus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methylococcus capsulatus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M MES (pH 6.5) and 15% PEG 20000 (w/v) | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | P22869 and P18798 and P11987 | alpha subunit and beta subunit and gamma subunit | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, Q sepharose column chromatography, S-200 gel filtration, and S-300 gel filtration | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propylene + NADH + H+ + O2 | - |
Methylococcus capsulatus | propylene oxide + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | - |
Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
MMOH | - |
Methylococcus capsulatus |
sMMO | the enzyme contains three protein components, a 251 kDa hydroxylase (MMOH), a 38.6 kDa reductase (MMOR), and a 15.9 kDa regulatory protein (MMOB) required to couple electron consumption with substrate hydroxylation at the catalytic diiron center of MMOH | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus |