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Literature summary for 1.14.13.25 extracted from

  • Tinberg, C.E.; Lippard, S.J.
    Oxidation reactions performed by soluble methane monooxygenase hydroxylase intermediates H(peroxo) and Q proceed by distinct mechanisms (2010), Biochemistry, 49, 7902-7912.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.25

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics for class III substrates of Hperoxo, and kinetics and thermodynamic parameters for class III substrates of Q at 4°C and 20°C, overview Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Methylococcus capsulatus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + NADH + H+ + O2 Methylococcus capsulatus
-
 methanol + H2O + NAD+
-
 ?
additional information Methylococcus capsulatus the enzyme catalyzes the selective oxidation of methane to methanol, but the enzyme is also capable of hydroxylating and epoxidizing a broad range of hydrocarbon substrates in addition to methane ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3 0.45 pH 7.0, 45°C Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NADH + H+ + O2
-
 Methylococcus capsulatus methanol + H2O + NAD+
-
 ?
methanol + NADH + H+ + O2 substrate of intermediate species, Hperoxo and Q, kinetics, overview Methylococcus capsulatus ? + H2O + NAD+
-
 ?
methylamine + NADH + H+ + O2 substrate of intermediate species, Hperoxo and Q, kinetics, overview Methylococcus capsulatus hydroxymethylamine + H2O + NAD+
-
 ?
methylcyanide + NADH + H+ + O2 substrate of intermediate species, Hperoxo and Q, kinetics, and proposed mechanism of CH3CN hydroxylation by Hperoxo, overview Methylococcus capsulatus hydroxymethylcyanide + H2O + NAD+
-
 ?
additional information the enzyme catalyzes the selective oxidation of methane to methanol, but the enzyme is also capable of hydroxylating and epoxidizing a broad range of hydrocarbon substrates in addition to methane Methylococcus capsulatus ?
-
 ?
additional information the enzyme catalyzes the selective oxidation of methane to methanol, but is also capable of hydroxylating and epoxidizing a broad range of hydrocarbon substrates in addition to methane. Reactions of the two intermediate species, of Hperoxo and Q, two oxidants that are generated sequentially during the reaction of reduced protein with O, with a panel of substrates of varying C-H bond strength, double-mixing stoppedflow spectroscopy, overview. Three classes of substrates exist according to the rate-determining step in the reaction Methylococcus capsulatus ?
-
 ?
propylaldehyde + NADH + H+ + O2 substrate of intermediate species, Hperoxo and Q, kinetics, overview Methylococcus capsulatus ? + H2O + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
MMO
-
 Methylococcus capsulatus
MMOH
-
 Methylococcus capsulatus
soluble methane monooxygenase
-
 Methylococcus capsulatus
soluble methane monooxygenase hydroxylase
-
 Methylococcus capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Methylococcus capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Methylococcus capsulatus

General Information

General Information Comment Organism
physiological function soluble methane monooxygenase is a bacterial enzyme that converts methane to methanol at a carboxylate-bridged diiron center with exquisite control. The enzyme is also capable of hydroxylating and epoxidizing a broad range of hydrocarbon substrates in addition to methane Methylococcus capsulatus