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Literature summary for 1.14.13.25 extracted from

  • Beauvais, L.G.; Lippard, S.J.
    Reactions of the diiron(IV) intermediate Q in soluble methane monooxygenase with fluoromethanes (2005), Biochem. Biophys. Res. Commun., 338, 262-266.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methylococcus capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information soluble enzyme, stopped-flow transient kinetics, double-mixing stopped-flow optical spectroscopic measurements, difluoromethane exhibits a smaller second-order rate constant for reaction with intermediate Q compared to methane Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + NADH + O2 Methylococcus capsulatus via diiron(IV) reaction intermediate Q, the decay rate of intermediate Q is substantially accelerated in the presence of fluuoromethane and difluoromethane methanol + NAD+ + H2O
-
?
methane + NADH + O2 Methylococcus capsulatus Bath via diiron(IV) reaction intermediate Q, the decay rate of intermediate Q is substantially accelerated in the presence of fluuoromethane and difluoromethane methanol + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-
Methylococcus capsulatus Bath
-
-
-

Reaction

Reaction Comment Organism Reaction ID
methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O via diiron(IV) reaction intermediate Q, reaction mechanism of the soluble enzyme with different substrates, overview Methylococcus capsulatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
difluoromethane + NADH + O2 soluble enzyme Methylococcus capsulatus difluoromethanol + NAD+ + H2O
-
?
difluoromethane + NADH + O2 soluble enzyme Methylococcus capsulatus Bath difluoromethanol + NAD+ + H2O
-
?
fluoromethane + NADH + O2 soluble enzyme Methylococcus capsulatus fluoromethanol + NAD+ + H2O
-
?
fluoromethane + NADH + O2 soluble enzyme Methylococcus capsulatus Bath fluoromethanol + NAD+ + H2O
-
?
methane + NADH + O2
-
Methylococcus capsulatus methanol + NAD+ + H2O
-
?
methane + NADH + O2 via diiron(IV) reaction intermediate Q, the decay rate of intermediate Q is substantially accelerated in the presence of fluuoromethane and difluoromethane Methylococcus capsulatus methanol + NAD+ + H2O
-
?
methane + NADH + O2
-
Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
?
methane + NADH + O2 via diiron(IV) reaction intermediate Q, the decay rate of intermediate Q is substantially accelerated in the presence of fluuoromethane and difluoromethane Methylococcus capsulatus Bath methanol + NAD+ + H2O
-
?
additional information fluoroform is no substrate Methylococcus capsulatus ?
-
?
additional information fluoroform is no substrate Methylococcus capsulatus Bath ?
-
?

Synonyms

Synonyms Comment Organism
MMO
-
Methylococcus capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Methylococcus capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylococcus capsulatus