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Literature summary for 1.14.13.25 extracted from
- Purification of a high specific activity methane monooxygenase hydroxylase component from a type II methanotroph (1988), Biochem. Biophys. Res. Commun., 154, 165-170.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains oxo-bridged binuclear iron clusters | Methylosinus trichosporium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme system consists of 3 protein components A, B, C | Methylosinus trichosporium |
| 240000 | - |
component A hydroxylase | Methylosinus trichosporium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylosinus trichosporium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hydroxylase component A | Methylosinus trichosporium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.764 | - |
all components, substrate propene | Methylosinus trichosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methane + NAD(P)H + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme system consists of 3 protein components A, B, C | Methylosinus trichosporium |
| More | component A is a hydroxylase | Methylosinus trichosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at, hydroxylase component | Methylosinus trichosporium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | turnover numbers of component A | Methylosinus trichosporium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Methylosinus trichosporium | |
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Release 2023.1 (January 2023)
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