General Stability | Organism |
---|---|
protein A unstable to successive freezing and thawing | Methylococcus capsulatus |
protein B, in crude form requires addition of protease inhibitor phenylmethylsulfonyl fluoride | Methylococcus capsulatus |
protein C requires presence of thiol protective agent, e.g. sodium thioglycolate throughout purification | Methylococcus capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetylene | - |
Methylococcus capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Methylococcus capsulatus | |
0.003 | - |
methane | - |
Methylococcus capsulatus | |
0.0168 | - |
O2 | - |
Methylococcus capsulatus | |
0.0558 | - |
NADH | with methane | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | enzyme from cells grown under conditions of low copper availability | Methylococcus capsulatus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | protein A, hydroxylase component: contains a binuclear iron center | Methylococcus capsulatus | |
Iron | protein C, reductase component: contains 1 [Fe2-S2] | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
see under subunits: molecular weights of the subunits of components | Methylococcus capsulatus |
additional information | - |
enzyme system consists of 3 protein components A, B, C | Methylococcus capsulatus |
17000 | - |
component: protein B, gel filtration | Methylococcus capsulatus |
38000 | 38550 | component: protein C, gel filtration | Methylococcus capsulatus |
220000 | - |
component: protein A, gel filtration | Methylococcus capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NAD(P)H + O2 | Methylococcus capsulatus | initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source | methanol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | Methylococcus capsulatus Bath | initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source | methanol + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
Bath | - |
Methylococcus capsulatus Bath | - |
Bath | - |
Purification (Comment) | Organism |
---|---|
- |
Methylococcus capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O | mechanism | Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Methylococcus capsulatus |
0.265 | - |
protein A after reconstitution of iron | Methylococcus capsulatus |
6 | - |
purified protein C | Methylococcus capsulatus |
11.37 | - |
purified protein B | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ammonia + NAD(P)H + O2 | - |
Methylococcus capsulatus | hydroxylamine + NAD(P)+ + H2O | - |
? | |
benzene + NAD(P)H + H+ + O2 | - |
Methylococcus capsulatus | phenol + hydroquinone + NAD(P)+ + H2O | - |
? | |
chloromethane + NAD(P)H + O2 | - |
Methylococcus capsulatus | formaldehyde + NAD(P)+ + H2O + ? | - |
? | |
CO + NAD(P)H + O2 | - |
Methylococcus capsulatus | CO2 + NAD(P)+ + H2O | - |
? | |
CO + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | CO2 + NAD(P)+ + H2O | - |
? | |
cyclohexane + NAD(P)H + O2 | - |
Methylococcus capsulatus | cyclohexanol + NAD(P)+ + H2O | - |
? | |
dichloromethane + NAD(P)H + O2 | - |
Methylococcus capsulatus | CO + Cl- + NAD(P)+ + H2O | - |
? | |
dimethyl ether + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + formaldehyde + NAD(P)+ + H2O | - |
? | |
dimethyl ether + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | methanol + formaldehyde + NAD(P)+ + H2O | - |
? | |
ethane + NAD(P)H + O2 | - |
Methylococcus capsulatus | ethanol + NAD(P)+ + H2O | - |
? | |
ethene + NAD(P)H + O2 | - |
Methylococcus capsulatus | epoxyethane + NAD(P)+ + H2O | - |
? | |
hexane + NAD(P)H + O2 | - |
Methylococcus capsulatus | 1-hexanol + 2-hexanol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source | Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source | Methylococcus capsulatus Bath | methanol + NAD(P)+ + H2O | - |
? | |
naphthalene + NAD(P)H + O2 | - |
Methylococcus capsulatus | alpha-naphthol + beta-naphthol + NAD(P)+ + H2O | - |
? | |
styrene + NAD(P)H + O2 | - |
Methylococcus capsulatus | styrene epoxide + NAD(P)+ + H2O | - |
? | |
styrene + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | styrene epoxide + NAD(P)+ + H2O | - |
? | |
trans-2-butene + NAD(P)H + O2 | - |
Methylococcus capsulatus | trans-2,3-epoxybutane + trans-2-buten-1-ol + NAD(P)+ + H2O | - |
? | |
trichloromethane + NAD(P)H + O2 | - |
Methylococcus capsulatus | CO2 + Cl- + NAD(P)+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | protein A: 2 * 54000-60630, alpha+ 2 * 42000-44720, beta + 2 * 17000-19840, gamma | Methylococcus capsulatus |
More | see under molecular weight for the size of the protein components | Methylococcus capsulatus |
More | enzyme system consists of 3 protein components A, B, C | Methylococcus capsulatus |
More | component A is a hydroxylase | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
MMO | - |
Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | protein C, reductase component: contains 1 mol FAD per mol protein | Methylococcus capsulatus | |
NADH | preferred | Methylococcus capsulatus | |
NADPH | - |
Methylococcus capsulatus |