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Literature summary for 1.14.13.25 extracted from

  • Pilkington, S.J.; Dalton, H.
    Soluble methane monooxygenase from Methylococcus capsulatus Bath (1990), Methods Enzymol., 188, 181-190.
No PubMed abstract available
Search for more literature for 1.14.13.25

General Stability

General Stability Organism
protein A unstable to successive freezing and thawing Methylococcus capsulatus
protein B, in crude form requires addition of protease inhibitor phenylmethylsulfonyl fluoride Methylococcus capsulatus
protein C requires presence of thiol protective agent, e.g. sodium thioglycolate throughout purification Methylococcus capsulatus

Inhibitors

Inhibitors Comment Organism Structure
Acetylene
-
 Methylococcus capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Methylococcus capsulatus
0.003
-
 methane
-
 Methylococcus capsulatus
0.0168
-
 O2
-
 Methylococcus capsulatus
0.0558
-
 NADH with methane Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm enzyme from cells grown under conditions of low copper availability Methylococcus capsulatus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron protein A, hydroxylase component: contains a binuclear iron center Methylococcus capsulatus
Iron protein C, reductase component: contains 1 [Fe2-S2] Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 see under subunits: molecular weights of the subunits of components Methylococcus capsulatus
additional information
-
 enzyme system consists of 3 protein components A, B, C Methylococcus capsulatus
17000
-
 component: protein B, gel filtration Methylococcus capsulatus
38000 38550 component: protein C, gel filtration Methylococcus capsulatus
220000
-
 component: protein A, gel filtration Methylococcus capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + NAD(P)H + O2 Methylococcus capsulatus initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source methanol + NAD(P)+ + H2O
-
 ?
methane + NAD(P)H + O2 Methylococcus capsulatus Bath initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source methanol + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
 Bath
-
Methylococcus capsulatus Bath
-
 Bath
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methylococcus capsulatus

Reaction

Reaction Comment Organism Reaction ID
methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O mechanism Methylococcus capsulatus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Methylococcus capsulatus
0.265
-
 protein A after reconstitution of iron Methylococcus capsulatus
6
-
 purified protein C Methylococcus capsulatus
11.37
-
 purified protein B Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ammonia + NAD(P)H + O2
-
 Methylococcus capsulatus hydroxylamine + NAD(P)+ + H2O
-
 ?
benzene + NAD(P)H + H+ + O2
-
 Methylococcus capsulatus phenol + hydroquinone + NAD(P)+ + H2O
-
 ?
chloromethane + NAD(P)H + O2
-
 Methylococcus capsulatus formaldehyde + NAD(P)+ + H2O + ?
-
 ?
CO + NAD(P)H + O2
-
 Methylococcus capsulatus CO2 + NAD(P)+ + H2O
-
 ?
CO + NAD(P)H + O2
-
 Methylococcus capsulatus Bath CO2 + NAD(P)+ + H2O
-
 ?
cyclohexane + NAD(P)H + O2
-
 Methylococcus capsulatus cyclohexanol + NAD(P)+ + H2O
-
 ?
dichloromethane + NAD(P)H + O2
-
 Methylococcus capsulatus CO + Cl- + NAD(P)+ + H2O
-
 ?
dimethyl ether + NAD(P)H + O2
-
 Methylococcus capsulatus methanol + formaldehyde + NAD(P)+ + H2O
-
 ?
dimethyl ether + NAD(P)H + O2
-
 Methylococcus capsulatus Bath methanol + formaldehyde + NAD(P)+ + H2O
-
 ?
ethane + NAD(P)H + O2
-
 Methylococcus capsulatus ethanol + NAD(P)+ + H2O
-
 ?
ethene + NAD(P)H + O2
-
 Methylococcus capsulatus epoxyethane + NAD(P)+ + H2O
-
 ?
hexane + NAD(P)H + O2
-
 Methylococcus capsulatus 1-hexanol + 2-hexanol + NAD(P)+ + H2O
-
 ?
methane + NAD(P)H + O2
-
 Methylococcus capsulatus methanol + NAD(P)+ + H2O
-
 ?
methane + NAD(P)H + O2 initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source Methylococcus capsulatus methanol + NAD(P)+ + H2O
-
 ?
methane + NAD(P)H + O2
-
 Methylococcus capsulatus Bath methanol + NAD(P)+ + H2O
-
 ?
methane + NAD(P)H + O2 initial step in the assimilation of methane in bacteria that grow with methane as sole carbon and energy source Methylococcus capsulatus Bath methanol + NAD(P)+ + H2O
-
 ?
naphthalene + NAD(P)H + O2
-
 Methylococcus capsulatus alpha-naphthol + beta-naphthol + NAD(P)+ + H2O
-
 ?
styrene + NAD(P)H + O2
-
 Methylococcus capsulatus styrene epoxide + NAD(P)+ + H2O
-
 ?
styrene + NAD(P)H + O2
-
 Methylococcus capsulatus Bath styrene epoxide + NAD(P)+ + H2O
-
 ?
trans-2-butene + NAD(P)H + O2
-
 Methylococcus capsulatus trans-2,3-epoxybutane + trans-2-buten-1-ol + NAD(P)+ + H2O
-
 ?
trichloromethane + NAD(P)H + O2
-
 Methylococcus capsulatus CO2 + Cl- + NAD(P)+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? protein A: 2 * 54000-60630, alpha+ 2 * 42000-44720, beta + 2 * 17000-19840, gamma Methylococcus capsulatus
More see under molecular weight for the size of the protein components Methylococcus capsulatus
More enzyme system consists of 3 protein components A, B, C Methylococcus capsulatus
More component A is a hydroxylase Methylococcus capsulatus

Synonyms

Synonyms Comment Organism
MMO
-
 Methylococcus capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
FAD protein C, reductase component: contains 1 mol FAD per mol protein Methylococcus capsulatus
NADH preferred Methylococcus capsulatus
NADPH
-
 Methylococcus capsulatus