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Literature summary for 1.14.13.25 extracted from

  • Tonge, G.M.; Harrison, D.E.F.; Higgins, I.J.
    Purification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b (1977), Biochem. J., 161, 333-344.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
no component of the enzyme is stable to freezing Methylosinus trichosporium

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dichloro-(6-phenylphenoxy)ethylamine hydrochloride
-
Methylosinus trichosporium
2,4-Dichloro-(6-phenylphenoxy)ethyldiethylamine
-
Methylosinus trichosporium
2-mercaptoethanol
-
Methylosinus trichosporium
3-Amino-1,2,4-triazole
-
Methylosinus trichosporium
Allylthiourea
-
Methylosinus trichosporium
Dimercaptopropanol
-
Methylosinus trichosporium
dithiothreitol
-
Methylosinus trichosporium
KCN
-
Methylosinus trichosporium
o-phenanthroline
-
Methylosinus trichosporium
SKF 525A
-
Methylosinus trichosporium
Thioacetamide
-
Methylosinus trichosporium
Thiosemicarbazide
-
Methylosinus trichosporium
Thiourea
-
Methylosinus trichosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
methane
-
Methylosinus trichosporium

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ copper-containing protein component contains one copper atom per molecule Methylosinus trichosporium
Cu2+ cytochrome component contains 0.3-0.8 atoms copper per molecule Methylosinus trichosporium
Iron cytochrome component contains 1 atom iron per molecule Methylosinus trichosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
see under subunits: molecular weights of the subunits of components Methylosinus trichosporium
additional information
-
3 components: 1 soluble CO-binding cytochrome c, 1 copper-containing protein, and 1 small protein, SDS-PAGE Methylosinus trichosporium
additional information
-
enzyme system consists of 3 protein components A, B, C Methylosinus trichosporium
9400
-
component: small protein, native PAGE Methylosinus trichosporium
13000
-
component: CO-binding cytochrome c, native PAGE Methylosinus trichosporium
47000
-
component: copper-containing protein, native PAGE Methylosinus trichosporium

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3 components: a soluble CO-binding cytochrome c, a copper-containing protein, another small protein Methylosinus trichosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Methylosinus trichosporium
6
-
-
Methylosinus trichosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butane + NAD(P)H + O2
-
Methylosinus trichosporium 1-butanol + 2-butanol + NAD(P)+ + H2O
-
?
CO + NAD(P)H + O2
-
Methylosinus trichosporium CO2 + NAD(P)+ + H2O
-
?
dimethyl ether + NAD(P)H + O2 no activity Methylosinus trichosporium methanol + formaldehyde + NAD(P)+ + H2O
-
?
ethane + NAD(P)H + O2
-
Methylosinus trichosporium ethanol + NAD(P)+ + H2O
-
?
methane + NAD(P)H + O2
-
Methylosinus trichosporium methanol + NAD(P)+ + H2O
-
?
propane + NAD(P)H + O2
-
Methylosinus trichosporium 1-propanol + 2-propanol + NAD(P)+ + H2O
-
?

Subunits

Subunits Comment Organism
?
-
Methylosinus trichosporium
More see under molecular weight for the size of the protein components Methylosinus trichosporium
More enzyme system consists of 3 protein components A, B, C Methylosinus trichosporium
More 3 components: 1 soluble CO-binding cytochrome c, 1 copper-containing protein, and 1 small protein, SDS-PAGE Methylosinus trichosporium
More component A is a hydroxylase Methylosinus trichosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9 7
-
Methylosinus trichosporium

pH Range

pH Minimum pH Maximum Comment Organism
6.4 7.4 pH 6.4: about 20% of activity maximum, pH 7.4: about 25% of activity maximum Methylosinus trichosporium

Cofactor

Cofactor Comment Organism Structure
cytochrome c one of three protein components is a soluble CO-binding cytochrome c Methylosinus trichosporium
NADH
-
Methylosinus trichosporium
NADPH
-
Methylosinus trichosporium