General Stability | Organism |
---|---|
no component of the enzyme is stable to freezing | Methylosinus trichosporium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-Dichloro-(6-phenylphenoxy)ethylamine hydrochloride | - |
Methylosinus trichosporium | |
2,4-Dichloro-(6-phenylphenoxy)ethyldiethylamine | - |
Methylosinus trichosporium | |
2-mercaptoethanol | - |
Methylosinus trichosporium | |
3-Amino-1,2,4-triazole | - |
Methylosinus trichosporium | |
Allylthiourea | - |
Methylosinus trichosporium | |
Dimercaptopropanol | - |
Methylosinus trichosporium | |
dithiothreitol | - |
Methylosinus trichosporium | |
KCN | - |
Methylosinus trichosporium | |
o-phenanthroline | - |
Methylosinus trichosporium | |
SKF 525A | - |
Methylosinus trichosporium | |
Thioacetamide | - |
Methylosinus trichosporium | |
Thiosemicarbazide | - |
Methylosinus trichosporium | |
Thiourea | - |
Methylosinus trichosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
methane | - |
Methylosinus trichosporium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | copper-containing protein component contains one copper atom per molecule | Methylosinus trichosporium | |
Cu2+ | cytochrome component contains 0.3-0.8 atoms copper per molecule | Methylosinus trichosporium | |
Iron | cytochrome component contains 1 atom iron per molecule | Methylosinus trichosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
see under subunits: molecular weights of the subunits of components | Methylosinus trichosporium |
additional information | - |
3 components: 1 soluble CO-binding cytochrome c, 1 copper-containing protein, and 1 small protein, SDS-PAGE | Methylosinus trichosporium |
additional information | - |
enzyme system consists of 3 protein components A, B, C | Methylosinus trichosporium |
9400 | - |
component: small protein, native PAGE | Methylosinus trichosporium |
13000 | - |
component: CO-binding cytochrome c, native PAGE | Methylosinus trichosporium |
47000 | - |
component: copper-containing protein, native PAGE | Methylosinus trichosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylosinus trichosporium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3 components: a soluble CO-binding cytochrome c, a copper-containing protein, another small protein | Methylosinus trichosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Methylosinus trichosporium |
6 | - |
- |
Methylosinus trichosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butane + NAD(P)H + O2 | - |
Methylosinus trichosporium | 1-butanol + 2-butanol + NAD(P)+ + H2O | - |
? | |
CO + NAD(P)H + O2 | - |
Methylosinus trichosporium | CO2 + NAD(P)+ + H2O | - |
? | |
dimethyl ether + NAD(P)H + O2 | no activity | Methylosinus trichosporium | methanol + formaldehyde + NAD(P)+ + H2O | - |
? | |
ethane + NAD(P)H + O2 | - |
Methylosinus trichosporium | ethanol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
propane + NAD(P)H + O2 | - |
Methylosinus trichosporium | 1-propanol + 2-propanol + NAD(P)+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | - |
Methylosinus trichosporium |
More | see under molecular weight for the size of the protein components | Methylosinus trichosporium |
More | enzyme system consists of 3 protein components A, B, C | Methylosinus trichosporium |
More | 3 components: 1 soluble CO-binding cytochrome c, 1 copper-containing protein, and 1 small protein, SDS-PAGE | Methylosinus trichosporium |
More | component A is a hydroxylase | Methylosinus trichosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7 | - |
Methylosinus trichosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.4 | pH 6.4: about 20% of activity maximum, pH 7.4: about 25% of activity maximum | Methylosinus trichosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | one of three protein components is a soluble CO-binding cytochrome c | Methylosinus trichosporium | |
NADH | - |
Methylosinus trichosporium | |
NADPH | - |
Methylosinus trichosporium |