Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.243 extracted from

  • Feingersch, R.; Shainsky, J.; Wood, T.K.; Fishman, A.
    Protein engineering of toluene monooxygenases for synthesis of chiral sulfoxides (2008), Appl. Environ. Microbiol., 74, 1555-1566 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.243

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Burkholderia cepacia

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of subunit structure Burkholderia cepacia

Protein Variants

Protein Variants Comment Organism
V106M mutation of the alpha-hydroxylase subunit TomA3, improves both rate and enantioselectivity. Mutant oxidizes methyl phenyl sulfide to the corresponding sulfoxide at a rate of 3.0 nmol/min/mg protein compared with 1.6 for the wild-type enzyme, and the enantiomeric excess (pro-S) increases from 51% for the wild type to 88% for the mutant Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 methyl p-tolyl sulfide wild-type, whole cell assay, recombinant protein, pH not specified in the publication, temperature not specified in the publication Burkholderia cepacia
0.129
-
 thioanisole wild-type, whole cell assay, recombinant protein, pH not specified in the publication, temperature not specified in the publication Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0
-
Burkholderia cepacia G4 Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9 Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl p-tolyl sulfide + NADH + H+ + O2
-
 Burkholderia cepacia methyl p-tolyl sulfoxide + NAD+ + H2O
-
 ?
methyl p-tolyl sulfide + NADH + H+ + O2
-
 Burkholderia cepacia G4 methyl p-tolyl sulfoxide + NAD+ + H2O
-
 ?
thioanisole + NADH + H+ + O2
-
 Burkholderia cepacia methyl phenyl sulfoxide + NAD+ + H2O
-
 ?
thioanisole + NADH + H+ + O2
-
 Burkholderia cepacia G4 methyl phenyl sulfoxide + NAD+ + H2O
-
 ?