Literature summary for 1.14.13.243 extracted from

Rui, L.; Kwon, Y.M.; Fishman, A.; Reardon, K.F.; Wood, T.K.
Saturation mutagenesis of toluene ortho-monooxygenase of Burkholderia cepacia G4 for enhanced 1-naphthol synthesis and chloroform degradation (2004), Appl. Environ. Microbiol., 70, 3246-3252 .

Search for more literature for 1.14.13.243

Cloned(Commentary)

Cloned (Comment)	Organism
-	Burkholderia cepacia

Protein Variants

Protein Variants	Comment	Organism
A106E	mutation in hydroxylase alpha-subunit TomA3, variant degrades its natural substrate toluene 63% faster than wild-type, with 50% 2-methylphenol, 25% 3-methylphenol, and 25% 4-methylphenol being formed. Whole cells expressing the A106E variant have two times better naphthalene-to-1-naphthol activity than the wild-type, and the regiospecificity of the A106E variant is unchanged, with 98% 1-naphthol formed	Burkholderia cepacia

Organism

Organism	UniProt	Comment	Textmining
Burkholderia cepacia	Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9	Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0	-
Burkholderia cepacia G4	Q9ANX4 and Q9ANX0 and Q9ANX3 and Q8VSV8 and Q9ANX1 and Q8VSV9	Q9ANX4 i.e. subunit tomA1, Q9ANX0 i.e. subunit tomA5, Q9ANX3 i.e. subunit TomA2, Q8VSV8 i.e. subunit TomA3, Q9ANX1 i.e. subunit TomA4, Q8VSV9 i.e. subunit TomA0	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
naphthalene + NADH + H+ + O2	-	Burkholderia cepacia	1-naphthol + NAD+ + H2O	-	?
naphthalene + NADH + H+ + O2	-	Burkholderia cepacia G4	1-naphthol + NAD+ + H2O	-	?
toluene + NADH + H+ + O2	-	Burkholderia cepacia	2-methylphenol + NAD+ + H2O	-	?
toluene + NADH + H+ + O2	-	Burkholderia cepacia G4	2-methylphenol + NAD+ + H2O	-	?

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)