Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-pyridoxic acid | competitive with 3-hydroxy-2-methylpyridine-5-carboxylate | Pseudomonas sp. | |
6-Methylnicotinate | - |
Pseudomonas sp. | |
6-methylnicotinic acid | competitive with 3-hydroxy-2-methylpyridine-5-carboxylate | Pseudomonas sp. | |
p-chloromercuribenzoate | 0.05 mM, quick and complete inhibition | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
166000 | - |
equilibrium sedimentation | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 | Pseudomonas sp. | inducible enzyme opens the pyridine ring during the metabolic degradation of vitamin B6 | 2-(acetamidomethylene)succinate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
MA-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + H+ + O2 = 2-(acetamidomethylidene)succinate + NAD(P)+ | reaction proceeds in a concerted fashion via a ternary complex of oxygenase, NADH and 3-hydroxy-2-methylpyridine-5-carboxylate | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 0.1% 2-mercaptoethanol, 1 month | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 | - |
Pseudomonas sp. | 2-(acetamidomethylene)succinate + NAD(P)+ | - |
? | |
3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 | inducible enzyme opens the pyridine ring during the metabolic degradation of vitamin B6 | Pseudomonas sp. | 2-(acetamidomethylene)succinate + NAD+ | - |
? | |
5-pyridoxic acid + NADH + O2 | - |
Pseudomonas sp. | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 2 mol of FAD per mol of enzyme | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. |