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Literature summary for 1.14.13.24 extracted from

  • Park, M.; Jeon, Y.; Jang, H.H.; Ro, H.S.; Park, W.; Madsen, E.L.; Jeon, C.O.
    Molecular and biochemical characterization of 3-hydroxybenzoate 6-hydroxylase from Polaromonas naphthalenivorans CJ2 (2007), Appl. Environ. Microbiol., 73, 5146-5152.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.24

Cloned(Commentary)

Cloned (Comment) Organism
orf2 or gene nagX, which does not encode salicylate 5-hydroxylase, but 3-hydroxybenzoate 6-hydroxylase, DNA and amino acid sequence determination and analysis, genetic organization, overview, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Polaromonas naphthalenivorans

Protein Variants

Protein Variants Comment Organism
additional information construction of a nagX knockout mutant of strain CJ2 defective in nagX, the mutant fails to grow on 3-hydroxybenzoate but grows normally on naphthalene Polaromonas naphthalenivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
 3-hydroxybenzoate pH 8.0, 30°C, recombinant NagX, with NADH Polaromonas naphthalenivorans
0.493
-
 NADH pH 8.0, 30°C, recombinant NagX Polaromonas naphthalenivorans
0.86
-
 NADPH pH 8.0, 30°C, recombinant NagX Polaromonas naphthalenivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 recombinant enzyme, gel filtration Polaromonas naphthalenivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxybenzoate + NAD(P)H + H+ + O2 Polaromonas naphthalenivorans the small catabolic gene cluster nagR2-orf2I''KL in strain CJ2 may be multifunctional and is essential for the degradation of 3-hydroxybenzoate gentisate + NAD(P)+ + H2O i.e. 2,5-dihydroxybenzoate ?

Organism

Organism UniProt Comment Textmining
Polaromonas naphthalenivorans
-
 orf2, gene nagX
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzoate + NAD(P)H + H+ + O2
-
 Polaromonas naphthalenivorans gentisate + NAD(P)+ + H2O i.e. 2,5-dihydroxybenzoate ?
3-hydroxybenzoate + NAD(P)H + H+ + O2 the small catabolic gene cluster nagR2-orf2I''KL in strain CJ2 may be multifunctional and is essential for the degradation of 3-hydroxybenzoate Polaromonas naphthalenivorans gentisate + NAD(P)+ + H2O i.e. 2,5-dihydroxybenzoate ?
3-hydroxybenzoate + NADH + H+ + O2
-
 Polaromonas naphthalenivorans 2,5-dihydroxybenzoate + NAD+ + H2O
-
 r
additional information the enzyme shows no salicylate 5-hydroxylase activity Polaromonas naphthalenivorans ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43000, recombinant enzyme, SDS-PAGE Polaromonas naphthalenivorans

Synonyms

Synonyms Comment Organism
3-hydroxybenzoate 6-hydroxylase
-
 Polaromonas naphthalenivorans
NagX
-
 Polaromonas naphthalenivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Polaromonas naphthalenivorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 inactive above 50°C Polaromonas naphthalenivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
 Polaromonas naphthalenivorans

pH Range

pH Minimum pH Maximum Comment Organism
5 9.4
-
 Polaromonas naphthalenivorans

Cofactor

Cofactor Comment Organism Structure
FAD bound to the enzyme Polaromonas naphthalenivorans
NADH
-
 Polaromonas naphthalenivorans
NADPH
-
 Polaromonas naphthalenivorans