Cloned (Comment) | Organism |
---|---|
gene FMOGS-OX2, quantitative real-time PCR enzyme expression analysis | Arabidopsis thaliana |
gene FMOGS-OX4, quantitative real-time PCR enzyme expression analysis | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an omega-(methylsulfanyl)alkyl-glucosinolate + NADPH + H+ + O2 | Arabidopsis thaliana | - |
an omega-(methylsulfinyl)alkyl-glucosinolate + NADP+ + H2O | - |
? | |
an omega-(methylsulfanyl)alkyl-glucosinolate + NADPH + H+ + O2 | Arabidopsis thaliana Col-0 | - |
an omega-(methylsulfinyl)alkyl-glucosinolate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q93Y23 | - |
- |
Arabidopsis thaliana | Q93Y23 | isoform FMOGS-Ox4 | - |
Arabidopsis thaliana | Q94K43 | - |
- |
Arabidopsis thaliana | Q94K43 | isoform FMOGS-Ox2 | - |
Arabidopsis thaliana Col-0 | Q93Y23 | - |
- |
Arabidopsis thaliana Col-0 | Q94K43 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | quantitative RT-PCR enzyme expression analysis in leaves and roots, overview | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
rosette leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an omega-(methylsulfanyl)alkyl-glucosinolate + NADPH + H+ + O2 | - |
Arabidopsis thaliana | an omega-(methylsulfinyl)alkyl-glucosinolate + NADP+ + H2O | - |
? | |
an omega-(methylsulfanyl)alkyl-glucosinolate + NADPH + H+ + O2 | - |
Arabidopsis thaliana Col-0 | an omega-(methylsulfinyl)alkyl-glucosinolate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavin-containing monooxygenase 2 | - |
Arabidopsis thaliana |
flavin-containing monooxygenase 4 | - |
Arabidopsis thaliana |
FMO2 | - |
Arabidopsis thaliana |
FMO4 | - |
Arabidopsis thaliana |
FMOGS-OX | - |
Arabidopsis thaliana |
FMOGS-OX2 | - |
Arabidopsis thaliana |
FMOGS-OX4 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | expression of isoforms FMOGS-OX2 and FMOGS-OX4 is induced by glucose treatment, independent of transcription factors MYB28/29 and MYC2/3/4. Glucose-triggered upregulation of FMOGS-OX2 and FMOGS-OX4 is partially regulated by abscisic acid through the key negative regulators ABI1 and ABI2, and the positive regulator SnRK2, but not via the transcription factor ABI5 | up |
Arabidopsis thaliana | the expression of FMOGS-OX2 and FMOGS-OX4 is induced by glucose treatment, independent of MYB28/29 and MYC2/3/4, the transcription factors that positively regulate aliphatic GSL biosynthesis. Glucose treatment of the abscisic acid (ABA)-related mutants indicated that glucose-triggered upregulation of FMOGS-OX2 and FMOGS-OX4 was partially regulated by ABA through the key negative regulators ABI1 and ABI2, and the positive regulator SnRK2, but not via the transcription factor ABI5. In wild-type plants, glucose treatment drastically reduces the accumulation of 4-methylthiobutyl (4MT) GSL, whereas a decrease in 4MT GSL is not observed in the fmogs-ox2, abi1-1, abi2-1, aba2-1, or aba3-1 mutants. This result indicates that the decreased accumulation of 4MT GSL by glucose treatment is attributed to upregulation of FMOGS-OX2 via the ABA signaling pathway. The expression of the FMOGS-OX2 and FMOGS-OX4 genes is induced by glucose treatment independent of the MYB and MYC transcription factors | up |
General Information | Comment | Organism |
---|---|---|
physiological function | in wild-type plants, glucose treatment drastically reduces the accumulation of 4-methylthiobutyl glucosinolate, which is not observed in the FMOGS-Ox2 mutant | Arabidopsis thaliana |
physiological function | the first step in side-chain modification of aliphatic GSLs is the S-oxygenation of the methylthioalkyl (MT) moiety to the methylsulfinylalkyl (MS) moiety. This reaction is catalyzed by flavin-containing monooxygenase (FMOGS-OX), which is encoded by seven genes in Arabidopsis thaliana | Arabidopsis thaliana |