Application | Comment | Organism |
---|---|---|
synthesis | synthesis of hydroxytyrosol, a phenol present in olives, via double hydroxylation of 2-phenylethanol employing toluene monooxygenases as biocatalysts | Burkholderia cepacia |
synthesis | synthesis of hydroxytyrosol, a phenol present in olives, via double hydroxylation of 2-phenylethanol employing toluene monooxygenases as biocatalysts. Mutant TmoA S395C shows a 15fold increase in 2-phenylethanol hydroxylation rate | Pseudomonas mendocina |
Protein Variants | Comment | Organism |
---|---|---|
I100A | mutant hydroxylates o-tyrosol, m-tyrosol and p-tyrosol to form hydroxytyrosol | Pseudomonas mendocina |
I100D | mutant hydroxylates m-tyrosol to form hydroxytyrosol | Pseudomonas mendocina |
I100G | mutant hydroxylates o-tyrosol, m-tyrosol and p-tyrosol to form hydroxytyrosol | Pseudomonas mendocina |
I100S | mutant hydroxylates o-tyrosol, m-tyrosol and p-tyrosol to form hydroxytyrosol | Pseudomonas mendocina |
I100V | mutant hydroxylates m-tyrosol to form hydroxytyrosol | Pseudomonas mendocina |
S395C | mutation in subunit TmoA, shows a 15fold increase in 2-phenylethanol hydroxylation rate | Pseudomonas mendocina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | Q9ANX2 | isoform TomA3 | - |
Burkholderia cepacia G4 | Q9ANX2 | isoform TomA3 | - |
Pseudomonas mendocina | Q00456 and Q00457 and Q00460 | Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH | - |
Pseudomonas mendocina KR1 | Q00456 and Q00457 and Q00460 | Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethanol + 2 NADH + 2 H+ + O2 | - |
Pseudomonas mendocina | 3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | reaction of mutants I100A, I100S, I100G | ? | |
2-phenylethanol + 2 NADH + 2 H+ + O2 | - |
Pseudomonas mendocina KR1 | 3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | reaction of mutants I100A, I100S, I100G | ? | |
2-phenylethanol + NADH + H+ + O2 | - |
Pseudomonas mendocina | m-tyrosol + p-tyrosol + NAD+ + H2O | 63% m-tyosol + 37% p-tyrosol | ? | |
2-phenylethanol + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | m-tyrosol + p-tyrosol + NAD+ + H2O | 63% m-tyosol + 37% p-tyrosol | ? | |
2-phenylethanol + NADH + H+ + O2 | - |
Burkholderia cepacia | o-tyrosol + m-tyrosol + NAD+ + H2O | 64% o-tyrosol + 36% m-tyrosol | ? | |
2-phenylethanol + NADH + H+ + O2 | - |
Burkholderia cepacia G4 | o-tyrosol + m-tyrosol + NAD+ + H2O | 64% o-tyrosol + 36% m-tyrosol | ? | |
m-tyrosol + 2 NADH + 2 H+ + O2 | - |
Burkholderia cepacia | 2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | 9% 2,3-dihydroxyphenylethanol | ? | |
m-tyrosol + 2 NADH + 2 H+ + O2 | - |
Burkholderia cepacia G4 | 2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | 9% 2,3-dihydroxyphenylethanol | ? | |
m-tyrosol + 2 NADH + 2 H+ + O2 | - |
Pseudomonas mendocina | 3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | mutants I100A, I100S, I100D, I100V and I100G hydroxylate m-tyrosol to form 3,4-dihydroxyphenylethanol with conversion of 55%, 50%, 2%, 3% and 18%, respectively | ? | |
m-tyrosol + 2 NADH + 2 H+ + O2 | - |
Pseudomonas mendocina KR1 | 3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | mutants I100A, I100S, I100D, I100V and I100G hydroxylate m-tyrosol to form 3,4-dihydroxyphenylethanol with conversion of 55%, 50%, 2%, 3% and 18%, respectively | ? | |
additional information | no substrates for wild-type: o-tyrosol, m-tyrosol, p-tyrosol | Burkholderia cepacia | ? | - |
? | |
additional information | no substrates for wild-type: o-tyrosol, m-tyrosol, p-tyrosol | Pseudomonas mendocina | ? | - |
? | |
additional information | no substrates for wild-type: o-tyrosol, m-tyrosol, p-tyrosol | Burkholderia cepacia G4 | ? | - |
? | |
additional information | no substrates for wild-type: o-tyrosol, m-tyrosol, p-tyrosol | Pseudomonas mendocina KR1 | ? | - |
? | |
o-tyrosol + 2 NADH + 2 H+ + O2 | - |
Pseudomonas mendocina | 2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | mutants I100A, I100S and I100G hydroxylate p-tyrosol to form 3,4-dihydroxyphenylethanol with 24%, 11% and 13% conversion, respectively, respectively | ? | |
o-tyrosol + 2 NADH + 2 H+ + O2 | - |
Pseudomonas mendocina KR1 | 2,3-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | mutants I100A, I100S and I100G hydroxylate p-tyrosol to form 3,4-dihydroxyphenylethanol with 24%, 11% and 13% conversion, respectively, respectively | ? | |
o-tyrosol + NADH + H+ + O2 | - |
Burkholderia cepacia | 2,3-dihydroxyphenylethanol + NAD+ + H2O | 83% 2,3-dihydroxyphenylethanol | ? | |
o-tyrosol + NADH + H+ + O2 | reaction of mutant S395C, 18% conversion in 44 h | Pseudomonas mendocina | 2,3-dihydroxyphenylethanol + NAD+ + H2O | - |
? | |
o-tyrosol + NADH + H+ + O2 | - |
Burkholderia cepacia G4 | 2,3-dihydroxyphenylethanol + NAD+ + H2O | 83% 2,3-dihydroxyphenylethanol | ? | |
p-tyrosol + 2 NADH + 2 H+ + O2 | - |
Burkholderia cepacia | 3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | 10% 3,4-dihydroxyphenylethanol | ? | |
p-tyrosol + 2 NADH + 2 H+ + O2 | - |
Pseudomonas mendocina | 3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | mutants I100A, I100S and I100G hydroxylate p-tyrosol to form 3,4-dihydroxyphenylethanol with 65%, 48% and 98% conversion, respectively, respectively | ? | |
p-tyrosol + 2 NADH + 2 H+ + O2 | - |
Burkholderia cepacia G4 | 3,4-dihydroxyphenylethanol + 2 NAD+ + 2 H2O | 10% 3,4-dihydroxyphenylethanol | ? |
Synonyms | Comment | Organism |
---|---|---|
TmoA | - |
Pseudomonas mendocina |
TOM | - |
Burkholderia cepacia |