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Literature summary for 1.14.13.236 extracted from

  • Nolan, L.C.; OConnor, K.E.
    Use of Pseudomonas mendocina, or recombinant Escherichia coli cells expressing toluene-4-monooxygenase, and a cell-free tyrosinase for the synthesis of 4-fluorocatechol from fluorobenzene (2007), Biotechnol. Lett., 29, 1045-1050.
    View publication on PubMed
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Application

Application Comment Organism
synthesis transformation of 1 mM fluorobenzene by whole cells gives a 52% yield of 4-fluorocatechol as a single product. The yield is improved 1.6fold by adding 10 mM ascorbic acid to the biotransformations Pseudomonas mendocina

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina Q00456 and Q00457 and Q00460 and Q00458 and Q00459 Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively, Q00458 i.e. Rieske-type ferredoxin T4MOC, Q00459 i.e. effector protein T4MOD
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Pseudomonas mendocina KR1 Q00456 and Q00457 and Q00460 and Q00458 and Q00459 Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively, Q00458 i.e. Rieske-type ferredoxin T4MOC, Q00459 i.e. effector protein T4MOD
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 fluorobenzene + NADH + H+ + O2
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 Pseudomonas mendocina 4-fluorophenol + 4-fluorocatechol + NAD+ + H2O whole-cell reaction, the predominant product is either 4-fluorophenol or 4-fluorocatechol depending on the ratio of biocatalyst to substrate concentration ?
2 fluorobenzene + NADH + H+ + O2
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 Pseudomonas mendocina KR1 4-fluorophenol + 4-fluorocatechol + NAD+ + H2O whole-cell reaction, the predominant product is either 4-fluorophenol or 4-fluorocatechol depending on the ratio of biocatalyst to substrate concentration ?