Application | Comment | Organism |
---|---|---|
synthesis | transformation of 1 mM fluorobenzene by whole cells gives a 52% yield of 4-fluorocatechol as a single product. The yield is improved 1.6fold by adding 10 mM ascorbic acid to the biotransformations | Pseudomonas mendocina |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas mendocina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mendocina | Q00456 and Q00457 and Q00460 and Q00458 and Q00459 | Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively, Q00458 i.e. Rieske-type ferredoxin T4MOC, Q00459 i.e. effector protein T4MOD | - |
Pseudomonas mendocina KR1 | Q00456 and Q00457 and Q00460 and Q00458 and Q00459 | Q00456, Q00457 an Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively, Q00458 i.e. Rieske-type ferredoxin T4MOC, Q00459 i.e. effector protein T4MOD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 fluorobenzene + NADH + H+ + O2 | - |
Pseudomonas mendocina | 4-fluorophenol + 4-fluorocatechol + NAD+ + H2O | whole-cell reaction, the predominant product is either 4-fluorophenol or 4-fluorocatechol depending on the ratio of biocatalyst to substrate concentration | ? | |
2 fluorobenzene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | 4-fluorophenol + 4-fluorocatechol + NAD+ + H2O | whole-cell reaction, the predominant product is either 4-fluorophenol or 4-fluorocatechol depending on the ratio of biocatalyst to substrate concentration | ? |