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Literature summary for 1.14.13.236 extracted from

  • Elsen, N.L.; Bailey, L.J.; Hauser, A.D.; Fox, B.G.
    Role for threonine 201 in the catalytic cycle of the soluble diiron hydroxylase toluene 4-monooxygenase (2009), Biochemistry, 48, 3838-3846.
    View publication on PubMed
Search for more literature for 1.14.13.236

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas mendocina

Crystallization (Commentary)

Crystallization (Comment) Organism
comparison of X-ray crystal structures of resting and reduced mutant T201A in complex with effector T4moD with structures of wild-type reveals changes in the positions of several key active site residues Pseudomonas mendocina

Protein Variants

Protein Variants Comment Organism
T201A mutation has no impact on steady-state catalysis or coupling. Mutant T201A gives stoichometric release of H2O2 during reaction in the absence of substrate and has a faster first-order rate constant for product formation than wild-type Pseudomonas mendocina
T201G mutation has no impact on steady-state catalysis or coupling Pseudomonas mendocina
T201S mutation has no impact on steady-state catalysis or coupling Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina Q00456 subunit TmoA
-
Pseudomonas mendocina KR1 Q00456 subunit TmoA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anisole + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methoxyphenol + NAD+ + H2O 87% efficiency ?
anisole + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methoxyphenol + NAD+ + H2O 87% efficiency ?
benzene + NADH + H+ + O2
-
 Pseudomonas mendocina phenol + NAD+ + H2O 98% efficiency ?
benzene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 phenol + NAD+ + H2O 98% efficiency ?
chlorobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-chlorophenol + NAD+ + H2O 39% efficiency ?
chlorobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-chlorophenol + NAD+ + H2O 39% efficiency ?
nitrobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-nitrophenol + NAD+ + H2O 12% efficiency ?
nitrobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-nitrophenol + NAD+ + H2O 12% efficiency ?
toluene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 194% efficiency ?
toluene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methylphenol + NAD+ + H2O 194% efficiency ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 nitrobenzene wild-type, pH 7.5, 4°C Pseudomonas mendocina
0.034
-
 nitrobenzene mutant T201A, pH 7.5, 4°C Pseudomonas mendocina
0.075
-
 chlorobenzene wild-type, pH 7.5, 4°C Pseudomonas mendocina
0.087
-
 chlorobenzene mutant T201A, pH 7.5, 4°C Pseudomonas mendocina
0.1
-
 Benzene wild-type, pH 7.5, 4°C Pseudomonas mendocina
0.16
-
 anisole wild-type, pH 7.5, 4°C Pseudomonas mendocina
0.21
-
 Toluene wild-type, pH 7.5, 4°C Pseudomonas mendocina
0.23
-
 Toluene mutant T201A, pH 7.5, 4°C Pseudomonas mendocina
0.23
-
 anisole mutant T201A, pH 7.5, 4°C Pseudomonas mendocina
0.31
-
 Benzene mutant T201A, pH 7.5, 4°C Pseudomonas mendocina

General Information

General Information Comment Organism
physiological function during reactions in the absence of substrate, wild-type toluene 4-monooxygenase hydroxylase T4moH slowly consumes O2 but only a negligible amount of H2O2 is released Pseudomonas mendocina