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Literature summary for 1.14.13.236 extracted from

  • Lountos, G.T.; Mitchell, K.H.; Studts, J.M.; Fox, B.G.; Orville, A.M.
    Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein (2005), Biochemistry, 44, 7131-7142.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures for native T4moD and variants with either four (DELTAN4-), or 10 (DELTAN10-) residues removed from the N-terminal at 2.1-, 1.7-, and 1.9 A resolution, respectively. Alterations of the N-terminal have little influence on the folded core of the protein Pseudomonas mendocina

Protein Variants

Protein Variants Comment Organism
additional information construction of variants with either four (DELTAN4-) seven (DELTAN7-), or 10 (DELTAN10-) residues removed from the N-terminal. Removal leads to statistically insignificant changes in kcat, KM, kcat/KM, and KI relative to the native protein. There is no significant change in the regiospecificity of toluene oxidation with any of the T4moD variants Pseudomonas mendocina

Inhibitors

Inhibitors Comment Organism Structure
TmoD effector protein Pseudomonas mendocina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
Toluene mutant DELTAN4, pH 7.5, 25°C Pseudomonas mendocina
0.0032
-
Toluene mutant DELTAN10, pH 7.5, 25°C Pseudomonas mendocina
0.0053
-
Toluene mutant DELTAN7, pH 7.5, 25°C Pseudomonas mendocina
0.007
-
Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina Q00459 subnit T4moD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
toluene + NADH + H+ + O2
-
Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 96.2% 4-methylphenol, 1.5% 3-methylphenol, 0.9% 2-methylphenol, 1.4% benzyl alcohol ?

Synonyms

Synonyms Comment Organism
T4moD
-
Pseudomonas mendocina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8
-
Toluene mutant DELTAN7, pH 7.5, 25°C Pseudomonas mendocina
3.1
-
Toluene mutant DELTAN10, pH 7.5, 25°C Pseudomonas mendocina
3.3
-
Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina
3.4
-
Toluene mutant DELTAN4, pH 7.5, 25°C Pseudomonas mendocina

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.098
-
TmoD wild-type, pH 7.5, 25°C Pseudomonas mendocina
0.106
-
TmoD mutant DELTAN4, pH 7.5, 25°C Pseudomonas mendocina
0.11
-
TmoD mutant DELTAN7, pH 7.5, 25°C Pseudomonas mendocina
0.116
-
TmoD mutant DELTAN10, pH 7.5, 25°C Pseudomonas mendocina

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
470
-
Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina
530
-
Toluene mutant DELTAN7, pH 7.5, 25°C Pseudomonas mendocina
970
-
Toluene mutant DELTAN10, pH 7.5, 25°C Pseudomonas mendocina
1470
-
Toluene mutant DELTAN4, pH 7.5, 25°C Pseudomonas mendocina