Crystallization (Comment) | Organism |
---|---|
crystal structures for native T4moD and variants with either four (DELTAN4-), or 10 (DELTAN10-) residues removed from the N-terminal at 2.1-, 1.7-, and 1.9 A resolution, respectively. Alterations of the N-terminal have little influence on the folded core of the protein | Pseudomonas mendocina |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of variants with either four (DELTAN4-) seven (DELTAN7-), or 10 (DELTAN10-) residues removed from the N-terminal. Removal leads to statistically insignificant changes in kcat, KM, kcat/KM, and KI relative to the native protein. There is no significant change in the regiospecificity of toluene oxidation with any of the T4moD variants | Pseudomonas mendocina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
TmoD | effector protein | Pseudomonas mendocina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
Toluene | mutant DELTAN4, pH 7.5, 25°C | Pseudomonas mendocina | |
0.0032 | - |
Toluene | mutant DELTAN10, pH 7.5, 25°C | Pseudomonas mendocina | |
0.0053 | - |
Toluene | mutant DELTAN7, pH 7.5, 25°C | Pseudomonas mendocina | |
0.007 | - |
Toluene | wild-type, pH 7.5, 25°C | Pseudomonas mendocina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mendocina | Q00459 | subnit T4moD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + NADH + H+ + O2 | - |
Pseudomonas mendocina | 4-methylphenol + NAD+ + H2O | 96.2% 4-methylphenol, 1.5% 3-methylphenol, 0.9% 2-methylphenol, 1.4% benzyl alcohol | ? |
Synonyms | Comment | Organism |
---|---|---|
T4moD | - |
Pseudomonas mendocina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
Toluene | mutant DELTAN7, pH 7.5, 25°C | Pseudomonas mendocina | |
3.1 | - |
Toluene | mutant DELTAN10, pH 7.5, 25°C | Pseudomonas mendocina | |
3.3 | - |
Toluene | wild-type, pH 7.5, 25°C | Pseudomonas mendocina | |
3.4 | - |
Toluene | mutant DELTAN4, pH 7.5, 25°C | Pseudomonas mendocina |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.098 | - |
TmoD | wild-type, pH 7.5, 25°C | Pseudomonas mendocina | |
0.106 | - |
TmoD | mutant DELTAN4, pH 7.5, 25°C | Pseudomonas mendocina | |
0.11 | - |
TmoD | mutant DELTAN7, pH 7.5, 25°C | Pseudomonas mendocina | |
0.116 | - |
TmoD | mutant DELTAN10, pH 7.5, 25°C | Pseudomonas mendocina |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
470 | - |
Toluene | wild-type, pH 7.5, 25°C | Pseudomonas mendocina | |
530 | - |
Toluene | mutant DELTAN7, pH 7.5, 25°C | Pseudomonas mendocina | |
970 | - |
Toluene | mutant DELTAN10, pH 7.5, 25°C | Pseudomonas mendocina | |
1470 | - |
Toluene | mutant DELTAN4, pH 7.5, 25°C | Pseudomonas mendocina |