Protein Variants | Comment | Organism |
---|---|---|
T201A | parameters similar to wild-type | Pseudomonas mendocina |
T201L | parameters similar to wild-type | Pseudomonas mendocina |
T201S | parameters similar to wild-type | Pseudomonas mendocina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mendocina | Q00456 and Q00457 and Q00460 | Q00456, Q00457 and Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively | - |
Pseudomonas mendocina KR1 | Q00456 and Q00457 and Q00460 | Q00456, Q00457 and Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,1-diethylcyclopropane + NADH + H+ + O2 | - |
Pseudomonas mendocina | ? + NAD+ + H2O | - |
? | |
1,1-diethylcyclopropane + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | ? + NAD+ + H2O | - |
? | |
1,1-dimethylcyclopropane + NADH + H+ + O2 | - |
Pseudomonas mendocina | ? + NAD+ + H2O | - |
? | |
1,1-dimethylcyclopropane + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | ? + NAD+ + H2O | - |
? | |
additional information | products are consistent with both radical rearrangement and cation ring expansion. Products show high-fidelity incorporation of an O-atom from O2 in the un-rearranged and radical-rearranged products, while the O-atom found in the cation ring-expansion products is predominantly obtained by reaction with H2O | Pseudomonas mendocina | ? | - |
? | |
additional information | products are consistent with both radical rearrangement and cation ring expansion. Products show high-fidelity incorporation of an O-atom from O2 in the un-rearranged and radical-rearranged products, while the O-atom found in the cation ring-expansion products is predominantly obtained by reaction with H2O | Pseudomonas mendocina KR1 | ? | - |
? | |
norcarane + NADH + H+ + O2 | - |
Pseudomonas mendocina | endo-2-norcaranol + exo-2-norcaranol + endo-3-norcaranol + NAD+ + H2O | 72% coupling, 47.5% endo-2-norcaranol, 39.2% exo-2- and endo-3-norcaranol, 8.8% exo-3-norcaranol | ? | |
norcarane + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | endo-2-norcaranol + exo-2-norcaranol + endo-3-norcaranol + NAD+ + H2O | 72% coupling, 47.5% endo-2-norcaranol, 39.2% exo-2- and endo-3-norcaranol, 8.8% exo-3-norcaranol | ? | |
toluene + NADH + H+ + O2 | - |
Pseudomonas mendocina | 4-methylphenol + NAD+ + H2O | 94% coupling | ? | |
toluene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | 4-methylphenol + NAD+ + H2O | 94% coupling | ? |
Synonyms | Comment | Organism |
---|---|---|
T4MOH | - |
Pseudomonas mendocina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | - |
norcarane | mutant T201S, pH 7.5, 25°C | Pseudomonas mendocina | |
0.4 | - |
norcarane | mutant T201A, pH 7.5, 25°C | Pseudomonas mendocina | |
0.46 | - |
norcarane | mutant T201L, pH 7.5, 25°C | Pseudomonas mendocina | |
0.49 | - |
norcarane | wild-type, pH 7.5, 25°C | Pseudomonas mendocina | |
1.9 | - |
Toluene | mutant T201L, pH 7.5, 25°C | Pseudomonas mendocina | |
1.9 | - |
Toluene | mutant T201S, pH 7.5, 25°C | Pseudomonas mendocina | |
2.3 | - |
Toluene | wild-type, pH 7.5, 25°C | Pseudomonas mendocina | |
2.3 | - |
Toluene | mutant T201A, pH 7.5, 25°C | Pseudomonas mendocina |