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Literature summary for 1.14.13.236 extracted from

  • Pikus, J.D.; Mitchell, K.H.; Studts, J.M.; McClay, K.; Steffan, R.J.; Fox, B.G.
    Threonine 201 in the diiron enzyme toluene 4-monooxygenase is not required for catalysis (2000), Biochemistry, 39, 791-799.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
T201A mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution Pseudomonas mendocina
T201F mutation causes a substantial shift in the product distribution, and gives o- and p-cresol in a 1:1 ratio Pseudomonas mendocina
T201G mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution Pseudomonas mendocina
T201K decrease in activity Pseudomonas mendocina
T201S mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution Pseudomonas mendocina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina
0.006
-
Toluene mutant T201A, pH 7.5, 25°C Pseudomonas mendocina
0.006
-
Toluene mutant T201S, pH 7.5, 25°C Pseudomonas mendocina
0.009
-
Toluene mutant T201G, pH 7.5, 25°C Pseudomonas mendocina

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 2.2 mol of Fe per mol of purified alphabetagamma protomer Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina Q00456 subunit TmoA
-
Pseudomonas mendocina KR1 Q00456 subunit TmoA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
toluene + NADH + H+ + O2
-
Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol plus 0.8% benzyl alcohol ?
toluene + NADH + H+ + O2
-
Pseudomonas mendocina KR1 4-methylphenol + NAD+ + H2O 96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol plus 0.8% benzyl alcohol ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
Toluene mutant T201G, pH 7.5, 25°C Pseudomonas mendocina
1
-
Toluene mutant T201A, pH 7.5, 25°C Pseudomonas mendocina
1.2
-
Toluene mutant T201S, pH 7.5, 25°C Pseudomonas mendocina
1.3
-
Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
70
-
Toluene mutant T201G, pH 7.5, 25°C Pseudomonas mendocina
170
-
Toluene mutant T201A, pH 7.5, 25°C Pseudomonas mendocina
200
-
Toluene mutant T201S, pH 7.5, 25°C Pseudomonas mendocina
300
-
Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina