Protein Variants | Comment | Organism |
---|---|---|
T201A | mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution | Pseudomonas mendocina |
T201F | mutation causes a substantial shift in the product distribution, and gives o- and p-cresol in a 1:1 ratio | Pseudomonas mendocina |
T201G | mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution | Pseudomonas mendocina |
T201K | decrease in activity | Pseudomonas mendocina |
T201S | mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution | Pseudomonas mendocina |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
Toluene | wild-type, pH 7.5, 25°C | Pseudomonas mendocina | |
0.006 | - |
Toluene | mutant T201A, pH 7.5, 25°C | Pseudomonas mendocina | |
0.006 | - |
Toluene | mutant T201S, pH 7.5, 25°C | Pseudomonas mendocina | |
0.009 | - |
Toluene | mutant T201G, pH 7.5, 25°C | Pseudomonas mendocina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 2.2 mol of Fe per mol of purified alphabetagamma protomer | Pseudomonas mendocina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas mendocina | Q00456 | subunit TmoA | - |
Pseudomonas mendocina KR1 | Q00456 | subunit TmoA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + NADH + H+ + O2 | - |
Pseudomonas mendocina | 4-methylphenol + NAD+ + H2O | 96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol plus 0.8% benzyl alcohol | ? | |
toluene + NADH + H+ + O2 | - |
Pseudomonas mendocina KR1 | 4-methylphenol + NAD+ + H2O | 96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol plus 0.8% benzyl alcohol | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
Toluene | mutant T201G, pH 7.5, 25°C | Pseudomonas mendocina | |
1 | - |
Toluene | mutant T201A, pH 7.5, 25°C | Pseudomonas mendocina | |
1.2 | - |
Toluene | mutant T201S, pH 7.5, 25°C | Pseudomonas mendocina | |
1.3 | - |
Toluene | wild-type, pH 7.5, 25°C | Pseudomonas mendocina |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70 | - |
Toluene | mutant T201G, pH 7.5, 25°C | Pseudomonas mendocina | |
170 | - |
Toluene | mutant T201A, pH 7.5, 25°C | Pseudomonas mendocina | |
200 | - |
Toluene | mutant T201S, pH 7.5, 25°C | Pseudomonas mendocina | |
300 | - |
Toluene | wild-type, pH 7.5, 25°C | Pseudomonas mendocina |