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Literature summary for 1.14.13.236 extracted from

  • Tao, Y.; Bentley, W.E.; Wood, T.K.
    Phenol and 2-naphthol production by toluene 4-monooxygenases using an aqueous/dioctyl phthalate system (2005), Appl. Microbiol. Biotechnol., 68, 614-621.
    View publication on PubMed
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Application

Application Comment Organism
synthesis conversion of benzene to phenol and naphthalene to 2-naphthol in a two-phase system using whole cells expressing wild-type toluene 4-monooxygenase and the alpha subunit variant TmoA I100A. With mutant I100A, the solubility of naphthalene is enhanced and the toxicity of the naphthols is prevented by the use of a water/dioctyl phthalate (80:20, vol%) system More than 99%2-naphthol is extracted to the dioctyl phthalate phase, dihydroxynaphthalene formation is prevented, 92% 2-naphthol is formed, and 12% naphthalene is converted. Using 50 vol% dioctyl phthalate, and wild-type T4MO, a 51% conversion of benzene is obtained and phenol is produced at a purity of 97% Pseudomonas mendocina

Organism

Organism UniProt Comment Textmining
Pseudomonas mendocina Q00456
-
-
Pseudomonas mendocina KR1 Q00456
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzene + NADH + H+ + O2
-
 Pseudomonas mendocina phenol + NAD+ + H2O
-
 ?
benzene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 phenol + NAD+ + H2O
-
 ?
naphthalene + NADH + H+ + O2
-
 Pseudomonas mendocina 2-naphthol + NAD+ + H2O
-
 ?
naphthalene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 2-naphthol + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
TmoA
-
 Pseudomonas mendocina
toluene-4-monooxygenase system protein A
-
 Pseudomonas mendocina