Application | Comment | Organism |
---|---|---|
analysis | construction of a sensitive fluorescent reporter system to detect and characterize the activity of enzymes that act upon the antibiotic, tetracycline and its derivatives. In this system, expression of the lux operon is regulated by the tetracycline repressor, TetR, which is expressed from the same plasmid under the control of an arabinose-inducible promoter. Addition of very low concentrations of tetracycline derivatives, well below growth inhibitory concentrations, result in luminescence production. Introduction of a plasmid expressing TetX, a tetracycline-inactivating enzyme, causes a marked loss in luminescence due to enzyme-mediated reduction in the intracellular tetracycline concentration | Bacteroides fragilis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacteroides fragilis |
Protein Variants | Comment | Organism |
---|---|---|
D311A | mutation in a highly conserved residue in the FAD-binding site, inactive | Bacteroides fragilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
doxycycline | presence of 0.0002% arabinose, pH not specified in the publication, temperature not specified in the publication | Bacteroides fragilis | |
0.11 | - |
chlorotetracycline | presence of 0.0002% arabinose, pH not specified in the publication, temperature not specified in the publication | Bacteroides fragilis | |
0.13 | - |
anhydrotetracycline | presence of 0.0002% arabinose, pH not specified in the publication, temperature not specified in the publication | Bacteroides fragilis | |
0.16 | - |
anhydrotetracycline | presence of 0.02% arabinose, pH not specified in the publication, temperature not specified in the publication | Bacteroides fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anhydrotetracycline + NADPH + H+ + O2 | - |
Bacteroides fragilis | 11a-hydroxyanhydrotetracycline + NADP+ + H2O | - |
? | |
chlorotetracycline + NADPH + H+ + O2 | - |
Bacteroides fragilis | 11a-hydroxychlorotetracycline + NADP+ + H2O | - |
? | |
doxycycline + NADPH + H+ + O2 | - |
Bacteroides fragilis | 11a-hydroxydoxycycline + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TetX | - |
Bacteroides fragilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
chlorotetracycline | presence of 0.0002% arabinose, pH not specified in the publication, temperature not specified in the publication | Bacteroides fragilis | |
0.4 | - |
anhydrotetracycline | presence of 0.02% arabinose, pH not specified in the publication, temperature not specified in the publication | Bacteroides fragilis | |
0.6 | - |
doxycycline | presence of 0.0002% arabinose, pH not specified in the publication, temperature not specified in the publication | Bacteroides fragilis | |
0.7 | - |
anhydrotetracycline | presence of 0.0002% arabinose, pH not specified in the publication, temperature not specified in the publication | Bacteroides fragilis |