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Literature summary for 1.14.13.22 extracted from
- The purification and properties of cyclohexanone oxygenase from Nocardia globerula CL1 and Acinetobacter NCIB 9871 (1976), Eur. J. Biochem., 63, 175-192.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| microdialysis method, saturated ammonium sulfate solution | Nocardia globerula |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Acinetobacter sp. |  |
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Nocardia globerula | |
| Bathocuproine | slight inhibition | Acinetobacter sp. | |
| Bathocuproine | slight inhibition | Nocardia globerula | |
| EDTA | - |
Acinetobacter sp. | |
| EDTA | - |
Nocardia globerula | |
| iodoacetamide | - |
Acinetobacter sp. | |
| iodoacetamide | - |
Nocardia globerula | |
| additional information | NADPH protects against sulfhydryl active agents | Acinetobacter sp. | |
| additional information | NADPH protects against sulfhydryl active agents | Nocardia globerula | |
| p-hydroxymercuribenzoate | - |
Acinetobacter sp. | |
| p-hydroxymercuribenzoate | - |
Nocardia globerula | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Nocardia globerula | |
| additional information | - |
additional information | - |
Acinetobacter sp. | |
| 0.0035 | - |
Cuprizone | - |
Acinetobacter sp. | |
| 0.006 | - |
cyclohexanone | - |
Acinetobacter sp. | |
| 0.009 | - |
4-methylcyclohexanone | - |
Acinetobacter sp. | |
| 0.0112 | - |
2-Hydroxycyclohexanone | - |
Acinetobacter sp. | |
| 0.012 | - |
2-methylcyclohexanone | - |
Acinetobacter sp. | |
| 0.14 | - |
Norcamphor | - |
Acinetobacter sp. | |
| 0.17 | - |
Cyclohexan-1,2-dione | - |
Acinetobacter sp. | |
| 0.2 | - |
Cycloheptanone | - |
Acinetobacter sp. | |
| 0.39 | - |
4-Hydroxycyclohexanone | - |
Acinetobacter sp. | |
| 0.64 | - |
dihydrocarvone | - |
Acinetobacter sp. | |
| 1 | - |
(+)-Camphor | - |
Acinetobacter sp. | |
| 1.8 | - |
Cyclooctanone | - |
Acinetobacter sp. | |
| 3.6 | - |
Cyclopentanone | - |
Acinetobacter sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
low speed sedimentation without reaching equilibrium | Nocardia globerula |
| 59000 | - |
low speed sedimentation without reaching equilibrium | Acinetobacter sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter sp. | - |
NCIB 9871 | - |
| Nocardia globerula | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| enzyme is reduced by visible light in presence of EDTA under anaerobic conditions | Nocardia globerula |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Nocardia globerula |
- |
Acinetobacter sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.9 | - |
purified enzyme | Nocardia globerula |
| 21 | - |
purified enzyme | Acinetobacter sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, pH 7.1 | Nocardia globerula |
| -25°C, pH 7.1 | Acinetobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-camphor + NADPH + O2 | - |
Nocardia globerula | ? | - |
? | |
| (+)-camphor + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
| (+)-dihydrocarvone + NADPH + O2 | - |
Nocardia globerula | ? | - |
? | |
| (+)-dihydrocarvone + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
| 2-hydroxycyclohexanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-3-hydroxycycloheptane + NADP+ + H2O | - |
? | |
| 2-hydroxycyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-3-hydroxycycloheptane + NADP+ + H2O | - |
? | |
| 2-methylcyclohexanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 2-methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
| 4-hydroxycyclohexanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-5-hydroxycycloheptane + NADP+ + H2O | - |
? | |
| 4-hydroxycyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-5-hydroxycycloheptane + NADP+ + H2O | - |
? | |
| 4-methylcyclohexanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-5-methylcycloheptane + NADP+ + H2O | - |
? | |
| 4-methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-5-methylcycloheptane + NADP+ + H2O | - |
? | |
| cuprizone + NADPH + O2 | - |
Nocardia globerula | ? | - |
? | |
| cuprizone + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
| cyclobutanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| cyclobutanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
| cycloheptanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| cycloheptanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
| cyclohexan-1,2-dione + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2,3-dioxo-cycloheptane + NADP+ + H2O | - |
? | |
| cyclohexan-1,2-dione + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2,3-dioxo-cycloheptane + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + O2 | - |
Nocardia globerula | 6-hexanolide + NADP+ + H2O | - |
? | |
| cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 6-hexanolide + NADP+ + H2O | product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane | ? | |
| cyclooctanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| cyclooctanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
| cyclopentanone + NADPH + O2 | - |
Nocardia globerula | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| cyclopentanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
| dihydrocarvone + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
| norcamphor + NADPH + O2 | - |
Nocardia globerula | ? | - |
? | |
| norcamphor + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Acinetobacter sp. |
| monomer | 1 * 58000-59000, SDS-PAGE | Nocardia globerula |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Nocardia globerula |
| 30 | - |
assay at | Acinetobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Nocardia globerula |
| 9 | - |
- |
Acinetobacter sp. |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Nocardia globerula | |
| FAD | reactivates apoenzyme | Acinetobacter sp. | |
| FMN | no activity with | Acinetobacter sp. | |
| NADPH | - |
Nocardia globerula | |
| NADPH | - |
Acinetobacter sp. | |
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