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Literature summary for 1.14.13.22 extracted from

  • Trower, M.K.; Buckland, R.M.; Griffin, M.
    Characterization of a cyclohexane hydrolase and a cyclohexanone mono-oxygenase from a cyclohexane-degrading Xanthobacter sp. (1985), Biochem. Soc. Trans., 13, 463-464.
No PubMed abstract available
Search for more literature for 1.14.13.22

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Pseudomonas sp. 5737
-
cytoplasm
-
 Xanthobacter sp. 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 1 * 51000, SDS-PAGE Xanthobacter sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Xanthobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xanthobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1
-
-
 Xanthobacter sp.

Storage Stability

Storage Stability Organism
-20°C, stable Xanthobacter sp.
4°C, in presence of cyclohexane or 0.1 mM NADPH Xanthobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phenyl-2-propanone + NADPH + O2
-
 Pseudomonas sp. ?
-
 ?
1-phenyl-2-propanone + NADPH + O2
-
 Xanthobacter sp. ?
-
 ?
2-phenyl-1-ethanal + NADPH + O2
-
 Pseudomonas sp. ?
-
 ?
2-phenyl-1-ethanal + NADPH + O2
-
 Xanthobacter sp. ?
-
 ?
cyclohexanone + NADPH + O2
-
 Xanthobacter sp. 6-hexanolide + NADP+ + H2O
-
 ?
additional information no activity towards straight-chain alkanones Pseudomonas sp. ?
-
 ?
additional information no activity towards straight-chain alkanones Xanthobacter sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 51000, SDS-PAGE Xanthobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas sp.
30
-
 assay at Xanthobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
 Xanthobacter sp.

Cofactor

Cofactor Comment Organism Structure
FMN 1 FMN molecule per molecule of protein Xanthobacter sp.
NADPH absolutely specific for NADPH as electron donor Pseudomonas sp.
NADPH absolutely specific for NADPH as electron donor Xanthobacter sp.