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Literature summary for 1.14.13.217 extracted from

  • Rodrigues, A.L.; Trachtmann, N.; Becker, J.; Lohanatha, A.F.; Blotenberg, J.; Bolten, C.J.; Korneli, C.; de Souza Lima, A.O.; Porto, L.M.; Sprenger, G.A.; Wittmann, C.
    Systems metabolic engineering of Escherichia coli for production of the antitumor drugs violacein and deoxyviolacein (2013), Metab. Eng., 20, 29-41.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene vioD, cloned under control of the PTAC promoter and inserted into the fuc locus of Escherichia coli strain BW25113, functional.expression of the vioABCE cluster from Chromobacterium violaceum strain ATCC 12472 under control of the inducible araC system and the vioD gene of Janthinobacterium lividum strain DSM 1522, which enables exclusive production of violacein in Escherichia coli K-12 MG1655 dVio-1 Janthinobacterium lividum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protodeoxyviolaceinate + NAD(P)H + H+ + O2 Janthinobacterium lividum
-
 protoviolaceinate + NAD(P)+
-
 ?
protodeoxyviolaceinate + NAD(P)H + H+ + O2 Janthinobacterium lividum DSM 1522
-
 protoviolaceinate + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Janthinobacterium lividum A1X1N6
-
-
Janthinobacterium lividum DSM 1522 A1X1N6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protodeoxyviolaceinate + NAD(P)H + H+ + O2
-
 Janthinobacterium lividum protoviolaceinate + NAD(P)+
-
 ?
protodeoxyviolaceinate + NAD(P)H + H+ + O2
-
 Janthinobacterium lividum DSM 1522 protoviolaceinate + NAD(P)+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Janthinobacterium lividum
NADPH
-
 Janthinobacterium lividum